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- W2092375882 abstract "Regeneration from nucellus calli was investigated to obtain basic data for somatic cell hybridization in apple Malus pumila Mill. In addition, isolation, culture and fusion of protoplasts derived from nucellus calli and leaves of the cultured shoots in vitro were investigated. The results are summarized as follows: 1. Calli derived from nucellus 50 days after anthesis of a cultivar ‘Fuji’ and a rootstock ‘CG 80’ produced adventitious embryos. Some of the embryos germinated and formed shoots after they were placed at 0°C in the dark for 6 months and then transferred to 25°C in the light. 2. The enzyme solutions containing 2% Cellulase Onozuka R-10 and 0.1% Pectolyase Y-23 or 2% Cellulase Onozuka RS and 0.1% Pectolyase Y-23 were the most effective for protoplast isolation from the nucellus calli and the leaves of shoots. The yield of protoplasts was the highest from the calli and the leaves of shoots cultured for 16- 20 days after transfer onto the new medium, except for a rootstock ‘M. 26’ where the highest yield was with 21-30 days of culture. The effective period of enzyme treatment on protoplast yield was 3.0-4.0h. Colony formation from protoplasts derived from nucellus calli was observed in all media used, except for the medium without growth regulators. 3. Somatic hybrid cells were produced by fusion of protoplasts isolated from the nucellus calli and the leaves of cultured shoots. Most of the hybrid protoplasts were identified by larger green protoplasts than the single green leaf protoplasts. The hybrid protoplasts and transparent nucellus callus protoplasts divided within 7-10 days of culture, while the single leaf protoplasts and the fused homokaryotic leaf protoplasts could not divide. The hybrid cells divided several times, and still had green chloroplasts. Successive cell division gave rise to cell colonies, and the green chloroplasts disappeared gradually. The colonies grew into calli which are, however, not identified as to whether they are hybrids." @default.
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- W2092375882 date "1989-01-01" @default.
- W2092375882 modified "2023-09-27" @default.
- W2092375882 title "Organ Formation from Calli and Protoplast Isolation, Culture, and Fusion in Apple, Malus pumila Mill" @default.
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- W2092375882 doi "https://doi.org/10.2503/jjshs.58.483" @default.
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