FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092446108> ?p ?o ?g. }

• W2092446108 endingPage "254" @default.
• W2092446108 startingPage "245" @default.
• W2092446108 abstract "Thyroid stimulating hormone (TSH), a pituitary glycoprotein hormone, is a potent inducer of intracellular cAMP production. Two methods for measuring TSH bioactivity were evaluated and compared. One assay is based on using a radioimmunoassay (RIA) to measure the recombinant human TSH-induced increase in cAMP using a bovine thyroid membrane isolate. The other is based on a Chinese hamster ovary (CHO) cell line that has been transfected with the TSH receptor and a cAMP-responsive luciferase reporter. The within-assay coefficient of variation for the membrane-based assay was determined to be approximately 35% compared with approximately 25% for the cell-based assay. Twenty-one preparations of recombinant human TSH (rhTSH) were tested using both methods. No significant difference was detected between the data sets and no assay bias was present. Both assay systems provide a suitable means for measuring the activity of rhTSH. The advantage of the membrane-based assay is the relatively small quantity of TSH needed for analysis. However, the average time required to analyse a sample using the membrane-based method was more than twice as long as that needed to test a sample in the cell-based assay. Other advantages of the cell-based method include the use of a 96-well format, which facilitates the analysis of several concentrations of rhTSH within one assay plate, and the use of a non-radioactive endpoint. Copyright 2002 The International Association for Biologicals. Published by Elsevier Science Ltd. All rights reserved." @default.
• W2092446108 created "2016-06-24" @default.
• W2092446108 creator A5001317694 @default.
• W2092446108 creator A5032086199 @default.
• W2092446108 creator A5049748981 @default.
• W2092446108 creator A5070153926 @default.
• W2092446108 creator A5083131465 @default.
• W2092446108 creator A5086529957 @default.
• W2092446108 creator A5091401518 @default.
• W2092446108 date "2002-09-01" @default.
• W2092446108 modified "2023-10-17" @default.
• W2092446108 title "Comparison of Two In Vitro Methods for the Measurement of Recombinant Human TSH Bioactivity" @default.
• W2092446108 cites W143943708 @default.
• W2092446108 cites W1547283546 @default.
• W2092446108 cites W1670588861 @default.
• W2092446108 cites W1971281264 @default.
• W2092446108 cites W1973500509 @default.
• W2092446108 cites W1974396708 @default.
• W2092446108 cites W1996479753 @default.
• W2092446108 cites W2008814445 @default.
• W2092446108 cites W2019386082 @default.
• W2092446108 cites W2019472675 @default.
• W2092446108 cites W2021756202 @default.
• W2092446108 cites W2031192188 @default.
• W2092446108 cites W2113537773 @default.
• W2092446108 cites W4233351214 @default.
• W2092446108 cites W4233743416 @default.
• W2092446108 cites W4253080431 @default.
• W2092446108 doi "https://doi.org/10.1006/biol.2002.0338" @default.
• W2092446108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12217348" @default.
• W2092446108 hasPublicationYear "2002" @default.
• W2092446108 type Work @default.
• W2092446108 sameAs 2092446108 @default.
• W2092446108 citedByCount "9" @default.
• W2092446108 countsByYear W20924461082013 @default.
• W2092446108 countsByYear W20924461082015 @default.
• W2092446108 countsByYear W20924461082018 @default.
• W2092446108 countsByYear W20924461082020 @default.
• W2092446108 countsByYear W20924461082023 @default.
• W2092446108 crossrefType "journal-article" @default.
• W2092446108 hasAuthorship W2092446108A5001317694 @default.
• W2092446108 hasAuthorship W2092446108A5032086199 @default.
• W2092446108 hasAuthorship W2092446108A5049748981 @default.
• W2092446108 hasAuthorship W2092446108A5070153926 @default.
• W2092446108 hasAuthorship W2092446108A5083131465 @default.
• W2092446108 hasAuthorship W2092446108A5086529957 @default.
• W2092446108 hasAuthorship W2092446108A5091401518 @default.
• W2092446108 hasConcept C104317684 @default.
• W2092446108 hasConcept C104488531 @default.
• W2092446108 hasConcept C111335760 @default.
• W2092446108 hasConcept C134018914 @default.
• W2092446108 hasConcept C153911025 @default.
• W2092446108 hasConcept C170493617 @default.
• W2092446108 hasConcept C175656101 @default.
• W2092446108 hasConcept C176685330 @default.
• W2092446108 hasConcept C185592680 @default.
• W2092446108 hasConcept C202751555 @default.
• W2092446108 hasConcept C2777886399 @default.
• W2092446108 hasConcept C40767141 @default.
• W2092446108 hasConcept C54009773 @default.
• W2092446108 hasConcept C54355233 @default.
• W2092446108 hasConcept C55493867 @default.
• W2092446108 hasConcept C71315377 @default.
• W2092446108 hasConcept C81885089 @default.
• W2092446108 hasConcept C86803240 @default.
• W2092446108 hasConceptScore W2092446108C104317684 @default.
• W2092446108 hasConceptScore W2092446108C104488531 @default.
• W2092446108 hasConceptScore W2092446108C111335760 @default.
• W2092446108 hasConceptScore W2092446108C134018914 @default.
• W2092446108 hasConceptScore W2092446108C153911025 @default.
• W2092446108 hasConceptScore W2092446108C170493617 @default.
• W2092446108 hasConceptScore W2092446108C175656101 @default.
• W2092446108 hasConceptScore W2092446108C176685330 @default.
• W2092446108 hasConceptScore W2092446108C185592680 @default.
• W2092446108 hasConceptScore W2092446108C202751555 @default.
• W2092446108 hasConceptScore W2092446108C2777886399 @default.
• W2092446108 hasConceptScore W2092446108C40767141 @default.
• W2092446108 hasConceptScore W2092446108C54009773 @default.
• W2092446108 hasConceptScore W2092446108C54355233 @default.
• W2092446108 hasConceptScore W2092446108C55493867 @default.
• W2092446108 hasConceptScore W2092446108C71315377 @default.
• W2092446108 hasConceptScore W2092446108C81885089 @default.
• W2092446108 hasConceptScore W2092446108C86803240 @default.
• W2092446108 hasIssue "3" @default.
• W2092446108 hasLocation W20924461081 @default.
• W2092446108 hasLocation W20924461082 @default.
• W2092446108 hasOpenAccess W2092446108 @default.
• W2092446108 hasPrimaryLocation W20924461081 @default.
• W2092446108 hasRelatedWork W2053393868 @default.
• W2092446108 hasRelatedWork W2057291960 @default.
• W2092446108 hasRelatedWork W2337696957 @default.
• W2092446108 hasRelatedWork W2354187767 @default.
• W2092446108 hasRelatedWork W2359175313 @default.
• W2092446108 hasRelatedWork W2367939407 @default.
• W2092446108 hasRelatedWork W2376295338 @default.
• W2092446108 hasRelatedWork W2378943006 @default.
• W2092446108 hasRelatedWork W2441498255 @default.
• W2092446108 hasRelatedWork W3031601676 @default.

• next