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- W2092480242 abstract "We report the first digital microfluidic (DMF) system capable of impedance sensing of mammalian cells. The new system was validated in three assays: calibration, proliferation, and serum sensing. In the first assay, three cell lines (HeLa, CHO-K1, and NIH-3T3) were seeded at different densities to determine the relationship between impedance and cell number, which was found to be linear for each type of cell. In the proliferation assay, cells were grown for four days and their proliferation rates were determined by regular impedance measurements. In the serum sensing assay, a dilution series of cell media containing different concentrations of serum was evaluated using impedance measurements to determine the optimum conditions for proliferation. The DMF impedance system is label-free, does not require imaging, and is compatible with long-term cell culture. We propose that this system will be useful for the growing number of scientists who are seeking methods other than fluorescence or cell sorting to analyze adherent cells in situ." @default.
- W2092480242 created "2016-06-24" @default.
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- W2092480242 date "2013-04-01" @default.
- W2092480242 modified "2023-10-08" @default.
- W2092480242 title "Digital microfluidics with impedance sensing for integrated cell culture andanalysis" @default.
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- W2092480242 doi "https://doi.org/10.1016/j.bios.2012.10.035" @default.
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