Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092506312> ?p ?o ?g. }
- W2092506312 endingPage "370" @default.
- W2092506312 startingPage "359" @default.
- W2092506312 abstract "The molecular identification of Nocardia species, when compared to phenotypic identification, has two primary advantages: rapid turn-around time and improved accuracy. The information content in the 5'-end of the 16S ribosomal RNA gene is sufficient for identification of most bacterial species. An evaluation was performed to demonstrate the quality of results provided by two specialized databases (RIDOM and MicroSeq 500 versions 1.1 and 1.4.3, library version 500-0125, respectively) and the more general GenBank database. In addition, these results were compared with phenotypic identifications. Partial 5'-16S rDNA sequences from 64 culture collection strains (DSM, CIP, JCM, and ATCC) were derived, in duplicate, independently in two laboratories. Furthermore, the sequences and the conventional identification results of 91 clinical Nocardia isolates were determined. With the exception of N. soli and N. cummidelens, all Nocardia type strains were distinguishable using 5'-16S rDNA sequencing. Assuming a normal distribution for the pairwise distances of all unique Nocardia sequences and choosing a reporting criterion of > or = 99.12% similarity for a distinct species, a statistical error probability of 1.0% can be calculated. When the various databases were searched with the clinical isolate sequences RIDOM gave a perfect match in 71.4% of cases whereas MicroSeq yielded a perfect match in only 26.4%. The GenBank service gave a 100% similarity in 59.3% but in 70.4% of these cases the results obtained were not exclusive for a single Nocardia species. Conventional methods gave a correct identification in 59 cases, although most recent taxonomic changes were not taken into account. The RIDOM service (http://www.ridom-rdna.de/) is in the process of making available a comprehensive and high-quality database for bacterial identification purposes and provides excellent results for the majority of Nocardia isolates." @default.
- W2092506312 created "2016-06-24" @default.
- W2092506312 creator A5010144593 @default.
- W2092506312 creator A5031312139 @default.
- W2092506312 creator A5041735503 @default.
- W2092506312 creator A5048705969 @default.
- W2092506312 creator A5062847820 @default.
- W2092506312 creator A5063415828 @default.
- W2092506312 creator A5083301912 @default.
- W2092506312 creator A5084682490 @default.
- W2092506312 date "2003-01-01" @default.
- W2092506312 modified "2023-09-27" @default.
- W2092506312 title "Evaluation of RIDOM, MicroSeq, and GenBank services in the molecular identification of Nocardia species" @default.
- W2092506312 cites W1543842283 @default.
- W2092506312 cites W1663403774 @default.
- W2092506312 cites W1675110385 @default.
- W2092506312 cites W1703862641 @default.
- W2092506312 cites W1858667347 @default.
- W2092506312 cites W1936926653 @default.
- W2092506312 cites W1949724122 @default.
- W2092506312 cites W1953726957 @default.
- W2092506312 cites W1971632686 @default.
- W2092506312 cites W1999844723 @default.
- W2092506312 cites W2018440767 @default.
- W2092506312 cites W2022921858 @default.
- W2092506312 cites W2033768130 @default.
- W2092506312 cites W2063496661 @default.
- W2092506312 cites W2065083813 @default.
- W2092506312 cites W2071619741 @default.
- W2092506312 cites W2088071473 @default.
- W2092506312 cites W2090932103 @default.
- W2092506312 cites W2092562663 @default.
- W2092506312 cites W2100887397 @default.
- W2092506312 cites W2101492502 @default.
- W2092506312 cites W2104417772 @default.
- W2092506312 cites W2104674971 @default.
- W2092506312 cites W2106882534 @default.
- W2092506312 cites W2108140555 @default.
- W2092506312 cites W2108554743 @default.
- W2092506312 cites W2111789213 @default.
- W2092506312 cites W2126321303 @default.
- W2092506312 cites W2133226036 @default.
- W2092506312 cites W2134755460 @default.
- W2092506312 cites W2141121101 @default.
- W2092506312 cites W2146711432 @default.
- W2092506312 cites W2151315112 @default.
- W2092506312 cites W2160004892 @default.
- W2092506312 cites W3188998055 @default.
- W2092506312 cites W4211022623 @default.
- W2092506312 cites W4246395620 @default.
- W2092506312 cites W4246729725 @default.
- W2092506312 cites W70486235 @default.
- W2092506312 doi "https://doi.org/10.1078/1438-4221-00271" @default.
- W2092506312 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14695064" @default.
- W2092506312 hasPublicationYear "2003" @default.
- W2092506312 type Work @default.
- W2092506312 sameAs 2092506312 @default.
- W2092506312 citedByCount "45" @default.
- W2092506312 countsByYear W20925063122012 @default.
- W2092506312 countsByYear W20925063122013 @default.
- W2092506312 countsByYear W20925063122014 @default.
- W2092506312 countsByYear W20925063122015 @default.
- W2092506312 countsByYear W20925063122016 @default.
- W2092506312 countsByYear W20925063122018 @default.
- W2092506312 countsByYear W20925063122019 @default.
- W2092506312 countsByYear W20925063122020 @default.
- W2092506312 countsByYear W20925063122022 @default.
- W2092506312 crossrefType "journal-article" @default.
- W2092506312 hasAuthorship W2092506312A5010144593 @default.
- W2092506312 hasAuthorship W2092506312A5031312139 @default.
- W2092506312 hasAuthorship W2092506312A5041735503 @default.
- W2092506312 hasAuthorship W2092506312A5048705969 @default.
- W2092506312 hasAuthorship W2092506312A5062847820 @default.
- W2092506312 hasAuthorship W2092506312A5063415828 @default.
- W2092506312 hasAuthorship W2092506312A5083301912 @default.
- W2092506312 hasAuthorship W2092506312A5084682490 @default.
- W2092506312 hasConcept C103278499 @default.
- W2092506312 hasConcept C104317684 @default.
- W2092506312 hasConcept C115961682 @default.
- W2092506312 hasConcept C116834253 @default.
- W2092506312 hasConcept C154945302 @default.
- W2092506312 hasConcept C2779448432 @default.
- W2092506312 hasConcept C41008148 @default.
- W2092506312 hasConcept C42062724 @default.
- W2092506312 hasConcept C523546767 @default.
- W2092506312 hasConcept C54355233 @default.
- W2092506312 hasConcept C59822182 @default.
- W2092506312 hasConcept C67905577 @default.
- W2092506312 hasConcept C70721500 @default.
- W2092506312 hasConcept C79029880 @default.
- W2092506312 hasConcept C86803240 @default.
- W2092506312 hasConceptScore W2092506312C103278499 @default.
- W2092506312 hasConceptScore W2092506312C104317684 @default.
- W2092506312 hasConceptScore W2092506312C115961682 @default.
- W2092506312 hasConceptScore W2092506312C116834253 @default.
- W2092506312 hasConceptScore W2092506312C154945302 @default.
- W2092506312 hasConceptScore W2092506312C2779448432 @default.
- W2092506312 hasConceptScore W2092506312C41008148 @default.