Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092510134> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2092510134 endingPage "806" @default.
- W2092510134 startingPage "792" @default.
- W2092510134 abstract "Using cultured islets as the experimental system, this study established dosage-response and time-dependency curves of the inductive glucose effect on glucose-stimulated insulin release, glucose usage, and glucokinase activity. Glucose-stimulated insulin release in islets cultured for 1, 2, or 7 days was increased as a function of glucose concentration in the culture medium and as a function of time. Glucose usage in the cultured islets showed a close relationship with glucose concentration in the culture medium at both 2 and 7 days of culture. Glucokinase activity increased in islets cultured for 1, 2, or 7 days as a function of increasing glucose concentrations in the culture medium and as a function of time. The Vmax of glucokinase in islets cultured for 7 days in medium containing 30 mM glucose was twice the value of freshly isolated islets and was almost fivefold higher than that in islets cultured for 7 days in 3 mM glucose. The glucose induction of glucose-stimulated insulin release, of glucose usage, and of glucokinase activity were tightly correlated. The biochemical mechanisms of glucose induction of islet glucokinase were further studied. Immunoblotting with an antibody against C-terminal peptide of glucokinase showed that densities of a 52,000-kD protein band from tissue extracts of islets cultured for 7 days in 3, 12, and 30 mM glucose were 25, 44, and 270% compared with that of extract from freshly isolated islets (100%). RNA blot analysis of glucokinase mRNA demonstrated virtually the same levels in fresh islets and islets after 7 days of culture in 3 or 30 mM glucose. The adaptive response of glucokinase to glucose appears therefore to be occurring at a translational or posttranslational site in cultured islets. These data greatly strengthen the concept that glucose is the regulator that induces the activity of glucokinase, which in turn determines the rate change of glucose usage as well as glucose-stimulated insulin release from β-cells. Thus, the hypothesis that glucokinase is the glucose sensor of β-cells is strengthened further." @default.
- W2092510134 created "2016-06-24" @default.
- W2092510134 creator A5000922713 @default.
- W2092510134 creator A5006197403 @default.
- W2092510134 creator A5018530141 @default.
- W2092510134 creator A5050182252 @default.
- W2092510134 creator A5050209319 @default.
- W2092510134 creator A5057271826 @default.
- W2092510134 creator A5085887181 @default.
- W2092510134 date "1992-07-01" @default.
- W2092510134 modified "2023-09-24" @default.
- W2092510134 title "Concordant Glucose Induction of Glucokinase, Glucose Usage, and Glucose-Stimulated Insulin Release in Pancreatic Islets Maintained in Organ Culture" @default.
- W2092510134 doi "https://doi.org/10.2337/diab.41.7.792" @default.
- W2092510134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1612193" @default.
- W2092510134 hasPublicationYear "1992" @default.
- W2092510134 type Work @default.
- W2092510134 sameAs 2092510134 @default.
- W2092510134 citedByCount "135" @default.
- W2092510134 countsByYear W20925101342012 @default.
- W2092510134 countsByYear W20925101342013 @default.
- W2092510134 countsByYear W20925101342014 @default.
- W2092510134 countsByYear W20925101342015 @default.
- W2092510134 countsByYear W20925101342016 @default.
- W2092510134 countsByYear W20925101342018 @default.
- W2092510134 countsByYear W20925101342019 @default.
- W2092510134 countsByYear W20925101342020 @default.
- W2092510134 countsByYear W20925101342021 @default.
- W2092510134 countsByYear W20925101342022 @default.
- W2092510134 crossrefType "journal-article" @default.
- W2092510134 hasAuthorship W2092510134A5000922713 @default.
- W2092510134 hasAuthorship W2092510134A5006197403 @default.
- W2092510134 hasAuthorship W2092510134A5018530141 @default.
- W2092510134 hasAuthorship W2092510134A5050182252 @default.
- W2092510134 hasAuthorship W2092510134A5050209319 @default.
- W2092510134 hasAuthorship W2092510134A5057271826 @default.
- W2092510134 hasAuthorship W2092510134A5085887181 @default.
- W2092510134 hasConcept C126322002 @default.
- W2092510134 hasConcept C134018914 @default.
- W2092510134 hasConcept C165220095 @default.
- W2092510134 hasConcept C17093226 @default.
- W2092510134 hasConcept C185592680 @default.
- W2092510134 hasConcept C2779306644 @default.
- W2092510134 hasConcept C2779855799 @default.
- W2092510134 hasConcept C2780963292 @default.
- W2092510134 hasConcept C71924100 @default.
- W2092510134 hasConcept C86803240 @default.
- W2092510134 hasConcept C98335778 @default.
- W2092510134 hasConceptScore W2092510134C126322002 @default.
- W2092510134 hasConceptScore W2092510134C134018914 @default.
- W2092510134 hasConceptScore W2092510134C165220095 @default.
- W2092510134 hasConceptScore W2092510134C17093226 @default.
- W2092510134 hasConceptScore W2092510134C185592680 @default.
- W2092510134 hasConceptScore W2092510134C2779306644 @default.
- W2092510134 hasConceptScore W2092510134C2779855799 @default.
- W2092510134 hasConceptScore W2092510134C2780963292 @default.
- W2092510134 hasConceptScore W2092510134C71924100 @default.
- W2092510134 hasConceptScore W2092510134C86803240 @default.
- W2092510134 hasConceptScore W2092510134C98335778 @default.
- W2092510134 hasIssue "7" @default.
- W2092510134 hasLocation W20925101341 @default.
- W2092510134 hasLocation W20925101342 @default.
- W2092510134 hasOpenAccess W2092510134 @default.
- W2092510134 hasPrimaryLocation W20925101341 @default.
- W2092510134 hasRelatedWork W1951162574 @default.
- W2092510134 hasRelatedWork W1989446301 @default.
- W2092510134 hasRelatedWork W1994481196 @default.
- W2092510134 hasRelatedWork W2020579285 @default.
- W2092510134 hasRelatedWork W2025398748 @default.
- W2092510134 hasRelatedWork W2030768859 @default.
- W2092510134 hasRelatedWork W2051290654 @default.
- W2092510134 hasRelatedWork W2060760400 @default.
- W2092510134 hasRelatedWork W2154711473 @default.
- W2092510134 hasRelatedWork W2889695791 @default.
- W2092510134 hasVolume "41" @default.
- W2092510134 isParatext "false" @default.
- W2092510134 isRetracted "false" @default.
- W2092510134 magId "2092510134" @default.
- W2092510134 workType "article" @default.