FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092521820> ?p ?o ?g. }

• W2092521820 endingPage "17311" @default.
• W2092521820 startingPage "17304" @default.
• W2092521820 abstract "The vesicle docking protein p115 showed saturable, high affinity binding to interphase Golgi membranes. The affinity of binding was up to 20-fold lower using membranes preincubated with mitotic cytosol. In contrast, binding was not affected by mitotic pretreatment of p115. The reduction in p115 binding was mediated by phosphorylation, could be induced by a cyclin-dependent kinase, and was fully reversible. A shift of p115 from membranes to cytosol was also found after fractionating mitotic cells. The functional significance of the decreased binding was addressed by in vitro mitotic incubations which disassemble Golgi cisternae, predominantly producing transport vesicles. The addition of excess p115 decreased loss of membrane from cisternae, indicating that p115's action is limiting while transport vesicles accumulate. The cessation of intra-Golgi traffic in mitosis has been hypothesized to result from an inhibition of membrane fusion while budding of transport vesicles continues. This process also contributes to mitotic Golgi disassembly. Our results imply that there is a mitotic modification to Golgi membranes leading to a reduction in the affinity of the p115 receptor. Reduced p115 binding may play a part in the inhibition of membrane fusion by preventing prior vesicle docking. The vesicle docking protein p115 showed saturable, high affinity binding to interphase Golgi membranes. The affinity of binding was up to 20-fold lower using membranes preincubated with mitotic cytosol. In contrast, binding was not affected by mitotic pretreatment of p115. The reduction in p115 binding was mediated by phosphorylation, could be induced by a cyclin-dependent kinase, and was fully reversible. A shift of p115 from membranes to cytosol was also found after fractionating mitotic cells. The functional significance of the decreased binding was addressed by in vitro mitotic incubations which disassemble Golgi cisternae, predominantly producing transport vesicles. The addition of excess p115 decreased loss of membrane from cisternae, indicating that p115's action is limiting while transport vesicles accumulate. The cessation of intra-Golgi traffic in mitosis has been hypothesized to result from an inhibition of membrane fusion while budding of transport vesicles continues. This process also contributes to mitotic Golgi disassembly. Our results imply that there is a mitotic modification to Golgi membranes leading to a reduction in the affinity of the p115 receptor. Reduced p115 binding may play a part in the inhibition of membrane fusion by preventing prior vesicle docking." @default.
• W2092521820 created "2016-06-24" @default.
• W2092521820 creator A5018833621 @default.
• W2092521820 creator A5045167388 @default.
• W2092521820 creator A5058516761 @default.
• W2092521820 creator A5073719453 @default.
• W2092521820 date "1996-07-01" @default.
• W2092521820 modified "2023-10-13" @default.
• W2092521820 title "Binding of the Vesicle Docking Protein p115 to Golgi Membranes Is Inhibited under Mitotic Conditions" @default.
• W2092521820 cites W1274298899 @default.
• W2092521820 cites W1432003149 @default.
• W2092521820 cites W1552784036 @default.
• W2092521820 cites W1563102716 @default.
• W2092521820 cites W1565222183 @default.
• W2092521820 cites W1575059414 @default.
• W2092521820 cites W1589866689 @default.
• W2092521820 cites W1623300751 @default.
• W2092521820 cites W185955435 @default.
• W2092521820 cites W1968006498 @default.
• W2092521820 cites W1971016259 @default.
• W2092521820 cites W1980137406 @default.
• W2092521820 cites W1984646224 @default.
• W2092521820 cites W1986035193 @default.
• W2092521820 cites W1988020006 @default.
• W2092521820 cites W1994225390 @default.
• W2092521820 cites W2000142886 @default.
• W2092521820 cites W2014868871 @default.
• W2092521820 cites W2016266703 @default.
• W2092521820 cites W2016417523 @default.
• W2092521820 cites W2021272662 @default.
• W2092521820 cites W2022772974 @default.
• W2092521820 cites W2029327566 @default.
• W2092521820 cites W2030623357 @default.
• W2092521820 cites W2032619982 @default.
• W2092521820 cites W2035124140 @default.
• W2092521820 cites W2040792888 @default.
• W2092521820 cites W2050753155 @default.
• W2092521820 cites W2057163724 @default.
• W2092521820 cites W2060526310 @default.
• W2092521820 cites W2064248967 @default.
• W2092521820 cites W2065015815 @default.
• W2092521820 cites W2069553229 @default.
• W2092521820 cites W2071629435 @default.
• W2092521820 cites W2084456466 @default.
• W2092521820 cites W2085680244 @default.
• W2092521820 cites W2089528428 @default.
• W2092521820 cites W2092507742 @default.
• W2092521820 cites W2094614586 @default.
• W2092521820 cites W2102948276 @default.
• W2092521820 cites W2119441048 @default.
• W2092521820 cites W2133798911 @default.
• W2092521820 cites W2136428115 @default.
• W2092521820 cites W2136822273 @default.
• W2092521820 cites W2137502154 @default.
• W2092521820 cites W2141799366 @default.
• W2092521820 cites W2152322252 @default.
• W2092521820 cites W2152791827 @default.
• W2092521820 cites W2153763976 @default.
• W2092521820 cites W2153924575 @default.
• W2092521820 cites W2154411920 @default.
• W2092521820 cites W2237943221 @default.
• W2092521820 cites W2270599862 @default.
• W2092521820 cites W253208710 @default.
• W2092521820 cites W4293247451 @default.
• W2092521820 doi "https://doi.org/10.1074/jbc.271.29.17304" @default.
• W2092521820 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8663393" @default.
• W2092521820 hasPublicationYear "1996" @default.
• W2092521820 type Work @default.
• W2092521820 sameAs 2092521820 @default.
• W2092521820 citedByCount "57" @default.
• W2092521820 countsByYear W20925218202012 @default.
• W2092521820 countsByYear W20925218202014 @default.
• W2092521820 countsByYear W20925218202015 @default.
• W2092521820 countsByYear W20925218202017 @default.
• W2092521820 countsByYear W20925218202018 @default.
• W2092521820 countsByYear W20925218202019 @default.
• W2092521820 countsByYear W20925218202020 @default.
• W2092521820 countsByYear W20925218202021 @default.
• W2092521820 countsByYear W20925218202022 @default.
• W2092521820 countsByYear W20925218202023 @default.
• W2092521820 crossrefType "journal-article" @default.
• W2092521820 hasAuthorship W2092521820A5018833621 @default.
• W2092521820 hasAuthorship W2092521820A5045167388 @default.
• W2092521820 hasAuthorship W2092521820A5058516761 @default.
• W2092521820 hasAuthorship W2092521820A5073719453 @default.
• W2092521820 hasBestOaLocation W20925218201 @default.
• W2092521820 hasConcept C119062480 @default.
• W2092521820 hasConcept C130316041 @default.
• W2092521820 hasConcept C158617107 @default.
• W2092521820 hasConcept C167019083 @default.
• W2092521820 hasConcept C181199279 @default.
• W2092521820 hasConcept C29452124 @default.
• W2092521820 hasConcept C41625074 @default.
• W2092521820 hasConcept C55493867 @default.
• W2092521820 hasConcept C86803240 @default.
• W2092521820 hasConcept C93304396 @default.
• W2092521820 hasConcept C95444343 @default.
• W2092521820 hasConcept C98539663 @default.

• next