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• W2092550995 abstract "Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (σB)-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes wild-type (wt) and ΔsigB backgrounds and then measuring (i) β-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the ΔsigB strain, confirming both growth phase- and σB-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by σB." @default.
• W2092550995 created "2016-06-24" @default.
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• W2092550995 date "2005-04-01" @default.
• W2092550995 modified "2023-10-17" @default.
• W2092550995 title "The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner" @default.
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• W2092550995 doi "https://doi.org/10.1016/j.femsle.2005.03.025" @default.
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