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• W2092607180 abstract "AI-2-mediated quorum sensing has been identified in various bacteria, including both Gram-negative and Gram-positive species, and numerous phenotypes have been reported to be regulated by this mechanism, using the luxS-mutant strain. But the AI-2 production process confused this regulatory function; some considered this regulation as the result of a metabolic change, which refers to an important metabolic cycle named activated methyl cycle (AMC), caused by luxS-mutant simultaneously with the defect of AI-2. Herein we hypothesized that the quorum sensing system--not the metabolic aspect--is responsible for such a regulatory function. In this study, we constructed plasmids infused with sahH and induced protein expression in the luxS-mutant strain to make the quorum-sensing system and metabolic system independent. The biofilm-related genes were investigated by real-time polymerase chain reaction (PCR), and the results demonstrated that the quorum-sensing completed strain restored the gene expression of the defective strain, but the metabolically completed one did not. This evidence supported our hypothesis that the autoinducer-2-mediated, quorum-sensing system, not the AMC, was responsible for luxS mutant regulation." @default.
• W2092607180 created "2016-06-24" @default.
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• W2092607180 date "2012-05-10" @default.
• W2092607180 modified "2023-10-03" @default.
• W2092607180 title "luxS Mutant Regulation: Quorum Sensing Impairment or Methylation Disorder?" @default.
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• W2092607180 doi "https://doi.org/10.3390/s120506176" @default.
• W2092607180 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3386735" @default.
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