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- W2092632251 abstract "This article contains detailed protocols for the localization of mRNA transcripts within whole Drosophila embryos. The procedures are based on the use of digoxigenin-, fluorescein-, and biotin-labeled antisense RNA probes for nonradioactive detection of transcripts. The labels are visualized in situ by differently colored water-insoluble precipitates using alkaline phosphatase- or beta-galactosidase-based immunoassays. First, a basic method is described that allows detection of transcript distribution(s) of one or more genes using the same color precipitate. Second, a sequential alkaline phosphatase detection method is presented that permits the visualization of two or three independent transcript patterns in multiple colors in the same embryo. Third, a shortened two-color in situ hybridization protocol is provided that employs a combination of beta-galactosidase and alkaline phosphatase colorimetric reactions for differential detection. The two-color in situ hybridization methods work equally well in Drosophila and zebrafish embryos and may therefore also be adaptable to other species." @default.
- W2092632251 created "2016-06-24" @default.
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- W2092632251 date "2001-04-01" @default.
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- W2092632251 title "One-, Two-, and Three-Color Whole-Mount in Situ Hybridization to Drosophila Embryos" @default.
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- W2092632251 doi "https://doi.org/10.1006/meth.2000.1148" @default.
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