Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092637827> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2092637827 endingPage "802" @default.
- W2092637827 startingPage "796" @default.
- W2092637827 abstract "Abstract NS0 cells are an important industrial cell line for the production of therapeutic monoclonal antibodies. Culturing these cells is challenging because they are cholesterol auxotrophs, and providing cholesterol to the cells is hampered by the low solubility of lipids in aqueous medium. Limited loading capacity, precipitation, instability, and toxicity are associated with traditional delivery methods that involve solvents or carrier molecules. In this work, nanoparticle cholesterol mixtures (NCM) were produced by electrohydrodynamic spraying and added directly to a cholesterol auxotroph NS0 cell line. Compared to a cholesterol‐cyclodextrin solution and a commercial proprietary cholesterol solution, SyntheChol™ NS0 supplement, NCM is significantly less cytotoxic. In the fed batch cell culture process, product titer was increased by 32% when the NCM supplement replaced SyntheChol™ NS0 supplement. An even greater product titer improvement, 64%, was achieved when both NCM and SyntheChol™ NS0 supplements were used in the fed‐batch process. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011" @default.
- W2092637827 created "2016-06-24" @default.
- W2092637827 creator A5002986938 @default.
- W2092637827 creator A5034056432 @default.
- W2092637827 creator A5050717173 @default.
- W2092637827 creator A5059356655 @default.
- W2092637827 creator A5069309658 @default.
- W2092637827 creator A5077991708 @default.
- W2092637827 date "2011-04-20" @default.
- W2092637827 modified "2023-10-12" @default.
- W2092637827 title "Enhanced productivity of NS0 cells in fed-batch culture with cholesterol nanoparticle supplementation" @default.
- W2092637827 cites W1496304716 @default.
- W2092637827 cites W1984675553 @default.
- W2092637827 cites W2019691746 @default.
- W2092637827 cites W2033852473 @default.
- W2092637827 cites W2036286392 @default.
- W2092637827 cites W2047804262 @default.
- W2092637827 cites W2054189154 @default.
- W2092637827 cites W2063396119 @default.
- W2092637827 cites W2063458939 @default.
- W2092637827 cites W2065266551 @default.
- W2092637827 cites W2082425815 @default.
- W2092637827 cites W2162250373 @default.
- W2092637827 cites W2342216575 @default.
- W2092637827 cites W4233249586 @default.
- W2092637827 cites W42465286 @default.
- W2092637827 cites W4707769 @default.
- W2092637827 doi "https://doi.org/10.1002/btpr.608" @default.
- W2092637827 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21509955" @default.
- W2092637827 hasPublicationYear "2011" @default.
- W2092637827 type Work @default.
- W2092637827 sameAs 2092637827 @default.
- W2092637827 citedByCount "6" @default.
- W2092637827 countsByYear W20926378272012 @default.
- W2092637827 countsByYear W20926378272013 @default.
- W2092637827 countsByYear W20926378272017 @default.
- W2092637827 countsByYear W20926378272018 @default.
- W2092637827 countsByYear W20926378272022 @default.
- W2092637827 crossrefType "journal-article" @default.
- W2092637827 hasAuthorship W2092637827A5002986938 @default.
- W2092637827 hasAuthorship W2092637827A5034056432 @default.
- W2092637827 hasAuthorship W2092637827A5050717173 @default.
- W2092637827 hasAuthorship W2092637827A5059356655 @default.
- W2092637827 hasAuthorship W2092637827A5069309658 @default.
- W2092637827 hasAuthorship W2092637827A5077991708 @default.
- W2092637827 hasConcept C159654299 @default.
- W2092637827 hasConcept C185592680 @default.
- W2092637827 hasConcept C203014093 @default.
- W2092637827 hasConcept C2778163477 @default.
- W2092637827 hasConcept C32611913 @default.
- W2092637827 hasConcept C542903549 @default.
- W2092637827 hasConcept C54355233 @default.
- W2092637827 hasConcept C55493867 @default.
- W2092637827 hasConcept C81885089 @default.
- W2092637827 hasConcept C86803240 @default.
- W2092637827 hasConceptScore W2092637827C159654299 @default.
- W2092637827 hasConceptScore W2092637827C185592680 @default.
- W2092637827 hasConceptScore W2092637827C203014093 @default.
- W2092637827 hasConceptScore W2092637827C2778163477 @default.
- W2092637827 hasConceptScore W2092637827C32611913 @default.
- W2092637827 hasConceptScore W2092637827C542903549 @default.
- W2092637827 hasConceptScore W2092637827C54355233 @default.
- W2092637827 hasConceptScore W2092637827C55493867 @default.
- W2092637827 hasConceptScore W2092637827C81885089 @default.
- W2092637827 hasConceptScore W2092637827C86803240 @default.
- W2092637827 hasIssue "3" @default.
- W2092637827 hasLocation W20926378271 @default.
- W2092637827 hasLocation W20926378272 @default.
- W2092637827 hasOpenAccess W2092637827 @default.
- W2092637827 hasPrimaryLocation W20926378271 @default.
- W2092637827 hasRelatedWork W1995553126 @default.
- W2092637827 hasRelatedWork W2020110316 @default.
- W2092637827 hasRelatedWork W2022756787 @default.
- W2092637827 hasRelatedWork W2115893501 @default.
- W2092637827 hasRelatedWork W2357378996 @default.
- W2092637827 hasRelatedWork W2603093586 @default.
- W2092637827 hasRelatedWork W4231587522 @default.
- W2092637827 hasRelatedWork W4243269481 @default.
- W2092637827 hasRelatedWork W4244400123 @default.
- W2092637827 hasRelatedWork W4246718725 @default.
- W2092637827 hasVolume "27" @default.
- W2092637827 isParatext "false" @default.
- W2092637827 isRetracted "false" @default.
- W2092637827 magId "2092637827" @default.
- W2092637827 workType "article" @default.