FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092658361> ?p ?o ?g. }

• W2092658361 abstract "Abstract Background Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs. Results Application of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching for the cause of the differential actin sensitivity of the two cell types, we analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was greatly enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. Conclusions We show that manipulating actin in specific cell types in somatic embryos using Lat B treatment accelerated and even synchronized the development of somatic embryos and may be of practical use in biotechnology." @default.
• W2092658361 created "2016-06-24" @default.
• W2092658361 creator A5007766385 @default.
• W2092658361 creator A5027713572 @default.
• W2092658361 creator A5034893132 @default.
• W2092658361 creator A5036964695 @default.
• W2092658361 creator A5055251361 @default.
• W2092658361 creator A5064797948 @default.
• W2092658361 creator A5066124626 @default.
• W2092658361 creator A5076681644 @default.
• W2092658361 creator A5077173292 @default.
• W2092658361 creator A5089966231 @default.
• W2092658361 date "2010-05-17" @default.
• W2092658361 modified "2023-10-09" @default.
• W2092658361 title "The role of actin isoforms in somatic embryogenesis in Norway spruce" @default.
• W2092658361 cites W107803781 @default.
• W2092658361 cites W149647281 @default.
• W2092658361 cites W1545247886 @default.
• W2092658361 cites W1550173105 @default.
• W2092658361 cites W1564176844 @default.
• W2092658361 cites W1610568548 @default.
• W2092658361 cites W1923070859 @default.
• W2092658361 cites W1965066617 @default.
• W2092658361 cites W1974813506 @default.
• W2092658361 cites W1978802793 @default.
• W2092658361 cites W1983175553 @default.
• W2092658361 cites W1991395782 @default.
• W2092658361 cites W1994424708 @default.
• W2092658361 cites W2002345609 @default.
• W2092658361 cites W2026311140 @default.
• W2092658361 cites W2032997275 @default.
• W2092658361 cites W2036802825 @default.
• W2092658361 cites W2057914392 @default.
• W2092658361 cites W2084591162 @default.
• W2092658361 cites W2085180312 @default.
• W2092658361 cites W2097098401 @default.
• W2092658361 cites W2097382368 @default.
• W2092658361 cites W2107827440 @default.
• W2092658361 cites W2110476295 @default.
• W2092658361 cites W2116089078 @default.
• W2092658361 cites W2117093778 @default.
• W2092658361 cites W2119704973 @default.
• W2092658361 cites W2152003598 @default.
• W2092658361 cites W2154284350 @default.
• W2092658361 cites W2165182802 @default.
• W2092658361 cites W2166055355 @default.
• W2092658361 cites W2203769065 @default.
• W2092658361 cites W4231338500 @default.
• W2092658361 cites W4241620377 @default.
• W2092658361 cites W50484279 @default.
• W2092658361 cites W97259155 @default.
• W2092658361 doi "https://doi.org/10.1186/1471-2229-10-89" @default.
• W2092658361 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3095357" @default.
• W2092658361 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20478025" @default.
• W2092658361 hasPublicationYear "2010" @default.
• W2092658361 type Work @default.
• W2092658361 sameAs 2092658361 @default.
• W2092658361 citedByCount "38" @default.
• W2092658361 countsByYear W20926583612012 @default.
• W2092658361 countsByYear W20926583612013 @default.
• W2092658361 countsByYear W20926583612014 @default.
• W2092658361 countsByYear W20926583612015 @default.
• W2092658361 countsByYear W20926583612016 @default.
• W2092658361 countsByYear W20926583612017 @default.
• W2092658361 countsByYear W20926583612018 @default.
• W2092658361 countsByYear W20926583612019 @default.
• W2092658361 countsByYear W20926583612020 @default.
• W2092658361 countsByYear W20926583612021 @default.
• W2092658361 countsByYear W20926583612023 @default.
• W2092658361 crossrefType "journal-article" @default.
• W2092658361 hasAuthorship W2092658361A5007766385 @default.
• W2092658361 hasAuthorship W2092658361A5027713572 @default.
• W2092658361 hasAuthorship W2092658361A5034893132 @default.
• W2092658361 hasAuthorship W2092658361A5036964695 @default.
• W2092658361 hasAuthorship W2092658361A5055251361 @default.
• W2092658361 hasAuthorship W2092658361A5064797948 @default.
• W2092658361 hasAuthorship W2092658361A5066124626 @default.
• W2092658361 hasAuthorship W2092658361A5076681644 @default.
• W2092658361 hasAuthorship W2092658361A5077173292 @default.
• W2092658361 hasAuthorship W2092658361A5089966231 @default.
• W2092658361 hasBestOaLocation W20926583611 @default.
• W2092658361 hasConcept C104317684 @default.
• W2092658361 hasConcept C125705527 @default.
• W2092658361 hasConcept C134305767 @default.
• W2092658361 hasConcept C142669718 @default.
• W2092658361 hasConcept C1491633281 @default.
• W2092658361 hasConcept C190062978 @default.
• W2092658361 hasConcept C196843134 @default.
• W2092658361 hasConcept C201468095 @default.
• W2092658361 hasConcept C2993400109 @default.
• W2092658361 hasConcept C53345823 @default.
• W2092658361 hasConcept C54355233 @default.
• W2092658361 hasConcept C86803240 @default.
• W2092658361 hasConcept C87073359 @default.
• W2092658361 hasConcept C93042943 @default.
• W2092658361 hasConcept C95444343 @default.
• W2092658361 hasConceptScore W2092658361C104317684 @default.
• W2092658361 hasConceptScore W2092658361C125705527 @default.
• W2092658361 hasConceptScore W2092658361C134305767 @default.
• W2092658361 hasConceptScore W2092658361C142669718 @default.

• next