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• W2092671946 abstract "Background. In vitro, tissue culture-associated differentiation assays have facilitated the identification of multiple tumor-cell types. Methods. We have investigated the capability of differentiation of three extraosseous Ewing's sarcoma cell lines toward a neural and muscular direction by in vitro stimulation with dibutyryl cyclic adenosinemonophosphate (db cAMP) and 5-azacytidine, respectively. Results. Elongation of cytoplasmic processes and increase of neural markers chromogranin, S-100 protein, and glial fibrillary acidic protein were observed after db cAMP treatment of these lines and neurosecretory granules as well as myelin figures were demonstrated ultrastructurally. These results support the existence of several pathways of neural differentiation in vitro—neuroblastic, Schwannian, and central glial—in stages of maturation more advanced than those previously reported in Ewing's sarcoma of bone. The cell lines showed no definitive myoblastic differentiation after 5-azacytidine treatment. Conclusions. These data suggest that these three extraosseous Ewing's sarcoma cell lines configurate a heterogeneous group of tumors with respect to capability of differentiation into the neural lineage, arrested at more advanced stages of neural crest development than Ewing's sarcoma of bone and without capability of myoblastic differentiation with 5-azacytidine." @default.
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• W2092671946 date "1994-02-01" @default.
• W2092671946 modified "2023-10-17" @default.
• W2092671946 title "Patterns of differentiation in extraosseous ewing's sarcoma cells. An in vitro study" @default.
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• W2092671946 doi "https://doi.org/10.1002/1097-0142(19940201)73:3<616::aid-cncr2820730319>3.0.co;2-j" @default.
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