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• W2092805183 abstract "We developed a real-time RT-PCR assay for the quantification of topoisomerase II (topo II) mRNA level. It was applied on peripheral leukaemic cells from 23 patients with acute myelogenous leukaemia (AML) and 23 with chronic lymphocytic leukaemia (CLL). RNA template dilutions from 0.25 to 25ng per reaction were used as standard curves for topo IIalpha, beta and the internal control 18S rRNA. About 57% (26/46) and 26% (12/46) of the specimens had detectable topo IIbeta and alpha mRNA, respectively. The correlation between these two factors was rho=0.7 and P=0.0001. No relationship between topo IIalpha or beta mRNA level and response to chemotherapy was found in AML patients (n=19 assessable for response). Our method is rapid and convenient for quantification of topo IIalpha and beta mRNA levels, and could be suitable for investigation in a larger population." @default.
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• W2092805183 date "2002-05-01" @default.
• W2092805183 modified "2023-10-02" @default.
• W2092805183 title "Real-time RT-PCR for the determination of topoisomerase II mRNA level in leukaemic cells" @default.
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• W2092805183 doi "https://doi.org/10.1016/s0145-2126(01)00144-8" @default.
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