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• W2092805468 abstract "Vitamin C is an antioxidant that can be considered a possible biomarker of oxidative stability in human milk. A high-performance liquid chromatographic method was developed and validated for determining the total Vitamin C (ascorbic acid and dehydroascorbic acid) and ascorbic acid levels in human milk. This method was then compared with an enzymatic method (a Colorimetric technique) for quantifying ascorbic acid levels. Repeatability and reproducibility were acceptable for all methods. However, the high-performance liquid chromatography (HPLC) technique provided more satisfactory results than the enzymatic method due to this last method detected 37% less ascorbic acid and does not determine the total Vitamin C because of the enzymatic method cannot reduce the dehydroascorbic acid (DHA) to ascorbic acid. Furthermore, the HPLC method has the added advantages that it requires less reagents and material, and is simpler and less time consuming than the enzymatic method. In conclusion, the drawbacks of this enzymatic method would justify its substitution for a HPLC method." @default.
• W2092805468 created "2016-06-24" @default.
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• W2092805468 date "2006-01-02" @default.
• W2092805468 modified "2023-10-01" @default.
• W2092805468 title "Rapid high-performance liquid chromatographic method for Vitamin C determination in human milk versus an enzymatic method" @default.
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• W2092805468 doi "https://doi.org/10.1016/j.jchromb.2005.10.018" @default.
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