Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092807609> ?p ?o ?g. }
- W2092807609 endingPage "12444" @default.
- W2092807609 startingPage "12434" @default.
- W2092807609 abstract "KDO8P synthase catalyzes the condensation of arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) to form the 8-carbon sugar KDO8P and inorganic phosphate (Pi). The X-ray structure of the wild-type enzyme shows that when both PEP and A5P bind, the active site becomes isolated from the environment due to a conformational change of the L7 loop. The structures of the R106G mutant, without substrates, and with PEP and PEP plus A5P bound, were determined and reveal that in R106G closure of the L7 loop is impaired. The structural perturbations originating from the loss of the Arg106 side chain point to a role of the L2 loop in stabilizing the closed conformation of the L7 loop. Despite the increased exposure of the R106G active site, no abnormal reaction of PEP with water was observed, ruling out the hypothesis that the primary function of the L7 loop is to shield the active site from bulk solvent during the condensation reaction. However, the R106G enzyme displays several kinetic abnormalities on both the substrate side (smaller KmPEP, larger KiA5P and KmA5P) and the product side (smaller KiPi and KiKDO8P) of the reaction. As a consequence, the mutant enzyme is less severely inhibited by A5P and more severely inhibited by Pi and KDO8P. Simulations of the flux of KDO8P synthesis under metabolic steady-state conditions (constant concentration of reactants and products over time) suggest that in vivo R106G is expected to perform optimally in a narrower range of substrate and product concentrations than the wild-type enzyme." @default.
- W2092807609 created "2016-06-24" @default.
- W2092807609 creator A5008902310 @default.
- W2092807609 creator A5031827734 @default.
- W2092807609 creator A5032764121 @default.
- W2092807609 creator A5043845885 @default.
- W2092807609 creator A5053314693 @default.
- W2092807609 creator A5065977345 @default.
- W2092807609 date "2005-08-26" @default.
- W2092807609 modified "2023-09-23" @default.
- W2092807609 title "The Catalytic and Conformational Cycle of <i>Aquifex aeolicus </i>KDO8P Synthase: Role of the L7 Loop<sup>,</sup>" @default.
- W2092807609 cites W1484451878 @default.
- W2092807609 cites W1530364531 @default.
- W2092807609 cites W1539796472 @default.
- W2092807609 cites W1543819219 @default.
- W2092807609 cites W1803956524 @default.
- W2092807609 cites W1817368947 @default.
- W2092807609 cites W1963861745 @default.
- W2092807609 cites W1971442785 @default.
- W2092807609 cites W1973553025 @default.
- W2092807609 cites W1975111853 @default.
- W2092807609 cites W1983752881 @default.
- W2092807609 cites W1988132884 @default.
- W2092807609 cites W1995004905 @default.
- W2092807609 cites W1999490833 @default.
- W2092807609 cites W2020429677 @default.
- W2092807609 cites W2025269211 @default.
- W2092807609 cites W2028916017 @default.
- W2092807609 cites W2033154191 @default.
- W2092807609 cites W2033735656 @default.
- W2092807609 cites W2034830756 @default.
- W2092807609 cites W2035429492 @default.
- W2092807609 cites W2038586634 @default.
- W2092807609 cites W2039665187 @default.
- W2092807609 cites W2040615655 @default.
- W2092807609 cites W2046696671 @default.
- W2092807609 cites W2047173579 @default.
- W2092807609 cites W2049114189 @default.
- W2092807609 cites W2061133426 @default.
- W2092807609 cites W2069784708 @default.
- W2092807609 cites W2078490780 @default.
- W2092807609 cites W2091042700 @default.
- W2092807609 cites W2133283923 @default.
- W2092807609 cites W2157829264 @default.
- W2092807609 cites W2171189187 @default.
- W2092807609 cites W2173272774 @default.
- W2092807609 cites W2339493109 @default.
- W2092807609 cites W33022696 @default.
- W2092807609 cites W4234317413 @default.
- W2092807609 cites W4246090862 @default.
- W2092807609 doi "https://doi.org/10.1021/bi051095q" @default.
- W2092807609 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4480877" @default.
- W2092807609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16156656" @default.
- W2092807609 hasPublicationYear "2005" @default.
- W2092807609 type Work @default.
- W2092807609 sameAs 2092807609 @default.
- W2092807609 citedByCount "13" @default.
- W2092807609 countsByYear W20928076092013 @default.
- W2092807609 countsByYear W20928076092014 @default.
- W2092807609 countsByYear W20928076092016 @default.
- W2092807609 countsByYear W20928076092018 @default.
- W2092807609 countsByYear W20928076092022 @default.
- W2092807609 crossrefType "journal-article" @default.
- W2092807609 hasAuthorship W2092807609A5008902310 @default.
- W2092807609 hasAuthorship W2092807609A5031827734 @default.
- W2092807609 hasAuthorship W2092807609A5032764121 @default.
- W2092807609 hasAuthorship W2092807609A5043845885 @default.
- W2092807609 hasAuthorship W2092807609A5053314693 @default.
- W2092807609 hasAuthorship W2092807609A5065977345 @default.
- W2092807609 hasBestOaLocation W20928076092 @default.
- W2092807609 hasConcept C104317684 @default.
- W2092807609 hasConcept C112243037 @default.
- W2092807609 hasConcept C114614502 @default.
- W2092807609 hasConcept C143937172 @default.
- W2092807609 hasConcept C181199279 @default.
- W2092807609 hasConcept C184670325 @default.
- W2092807609 hasConcept C185592680 @default.
- W2092807609 hasConcept C18903297 @default.
- W2092807609 hasConcept C2777289219 @default.
- W2092807609 hasConcept C2777339483 @default.
- W2092807609 hasConcept C2777400929 @default.
- W2092807609 hasConcept C28212737 @default.
- W2092807609 hasConcept C33923547 @default.
- W2092807609 hasConcept C41183919 @default.
- W2092807609 hasConcept C547475151 @default.
- W2092807609 hasConcept C55493867 @default.
- W2092807609 hasConcept C63338738 @default.
- W2092807609 hasConcept C71240020 @default.
- W2092807609 hasConcept C8010536 @default.
- W2092807609 hasConcept C86803240 @default.
- W2092807609 hasConceptScore W2092807609C104317684 @default.
- W2092807609 hasConceptScore W2092807609C112243037 @default.
- W2092807609 hasConceptScore W2092807609C114614502 @default.
- W2092807609 hasConceptScore W2092807609C143937172 @default.
- W2092807609 hasConceptScore W2092807609C181199279 @default.
- W2092807609 hasConceptScore W2092807609C184670325 @default.
- W2092807609 hasConceptScore W2092807609C185592680 @default.
- W2092807609 hasConceptScore W2092807609C18903297 @default.