FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2092810704> ?p ?o ?g. }

• W2092810704 endingPage "1585" @default.
• W2092810704 startingPage "1574" @default.
• W2092810704 abstract "ABSTRACT Pulsed-fieldgel electrophoresis (PFGE) is the most common genotypic method used in reference and clinical laboratories for typing methicillin-resistant Staphylococcus aureus (MRSA). Many different protocols have been developed in laboratories that have extensive experience with the technique and have established national databases. However, the comparabilities of the different European PFGE protocols for MRSA and of the various national MRSA clones themselves had not been addressed until now. This multinational European Union (EU) project has established for the first time a European database of representative epidemic MRSA (EMRSA) strains and has compared them by using a new “harmonized” PFGE protocol developed by a consensus approach that has demonstrated sufficient reproducibility to allow the successful comparison of pulsed-field gels between laboratories and the tracking of strains around the EU. In-house protocols from 10 laboratories in eight European countries were compared by each center with a “gold standard” or initial harmonized protocol in which many of the parameters had been standardized. The group found that it was not important to standardize some elements of the protocol, such as the type of agarose, DNA block preparation, and plug digestion. Other elements were shown to be critical, namely, a standard gel volume and concentration of agarose, the DNA concentration in the plug, the ionic strength and volume of running buffer used, the running temperature, the voltage, and the switching times of electrophoresis. A new harmonized protocol was agreed on, further modified in a pilot study in two laboratories, and finally tested by all others. Seven laboratories' gels were found to be of sufficiently good quality to allow comparison of the strains by using a computer software program, while two gels could not be analyzed because of inadequate destaining and DNA overloading. Good-quality gels and inclusion of an internal quality control strain are essential before attempting intercenter PFGE comparisons. A number of clonally related strains have been shown to be present in multiple countries throughout Europe. The well-known Iberian clone has been demonstrated in Belgium, Finland, France, Germany, and Spain (and from the wider HARMONY collection in Portugal, Slovenia, and Sweden). Strains from the United Kingdom (EMRSA-15 and -16) have been identified in several othercountries, and other clonally related strains have also been identified. This highlights the need for closer international collaboration to monitor the spread of current epidemic strains as well as the emergence of new ones." @default.
• W2092810704 created "2016-06-24" @default.
• W2092810704 creator A5000413148 @default.
• W2092810704 creator A5003646625 @default.
• W2092810704 creator A5004835104 @default.
• W2092810704 creator A5011001275 @default.
• W2092810704 creator A5013723014 @default.
• W2092810704 creator A5029611751 @default.
• W2092810704 creator A5030663532 @default.
• W2092810704 creator A5036098330 @default.
• W2092810704 creator A5038249679 @default.
• W2092810704 creator A5043434841 @default.
• W2092810704 creator A5043496834 @default.
• W2092810704 creator A5049743437 @default.
• W2092810704 creator A5051414592 @default.
• W2092810704 creator A5052580641 @default.
• W2092810704 creator A5054977892 @default.
• W2092810704 creator A5057000719 @default.
• W2092810704 creator A5058160584 @default.
• W2092810704 creator A5058959179 @default.
• W2092810704 creator A5059055660 @default.
• W2092810704 creator A5069162354 @default.
• W2092810704 creator A5069863581 @default.
• W2092810704 creator A5082432143 @default.
• W2092810704 creator A5084426405 @default.
• W2092810704 creator A5091093541 @default.
• W2092810704 date "2003-04-01" @default.
• W2092810704 modified "2023-10-10" @default.
• W2092810704 title "Harmonization of Pulsed-Field Gel Electrophoresis Protocols for Epidemiological Typing of Strains of Methicillin-Resistant <i>Staphylococcus aureus</i> : a Single Approach Developed by Consensus in 10 European Laboratories and Its Application for Tracing the Spread of Related Strains" @default.
• W2092810704 cites W1863772987 @default.
• W2092810704 cites W1899245726 @default.
• W2092810704 cites W1927169344 @default.
• W2092810704 cites W1955612312 @default.
• W2092810704 cites W1975231997 @default.
• W2092810704 cites W1989110465 @default.
• W2092810704 cites W2005853912 @default.
• W2092810704 cites W2054350590 @default.
• W2092810704 cites W2058331795 @default.
• W2092810704 cites W2059922522 @default.
• W2092810704 cites W2070702806 @default.
• W2092810704 cites W2081835782 @default.
• W2092810704 cites W2100233990 @default.
• W2092810704 cites W2103123638 @default.
• W2092810704 cites W2107315332 @default.
• W2092810704 cites W2107802728 @default.
• W2092810704 cites W2120804601 @default.
• W2092810704 cites W2154440790 @default.
• W2092810704 cites W2165374028 @default.
• W2092810704 cites W2165905044 @default.
• W2092810704 cites W2167240968 @default.
• W2092810704 cites W4237980918 @default.
• W2092810704 cites W68996462 @default.
• W2092810704 cites W71214772 @default.
• W2092810704 doi "https://doi.org/10.1128/jcm.41.4.1574-1585.2003" @default.
• W2092810704 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/153895" @default.
• W2092810704 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12682148" @default.
• W2092810704 hasPublicationYear "2003" @default.
• W2092810704 type Work @default.
• W2092810704 sameAs 2092810704 @default.
• W2092810704 citedByCount "630" @default.
• W2092810704 countsByYear W20928107042012 @default.
• W2092810704 countsByYear W20928107042013 @default.
• W2092810704 countsByYear W20928107042014 @default.
• W2092810704 countsByYear W20928107042015 @default.
• W2092810704 countsByYear W20928107042016 @default.
• W2092810704 countsByYear W20928107042017 @default.
• W2092810704 countsByYear W20928107042018 @default.
• W2092810704 countsByYear W20928107042019 @default.
• W2092810704 countsByYear W20928107042020 @default.
• W2092810704 countsByYear W20928107042021 @default.
• W2092810704 countsByYear W20928107042022 @default.
• W2092810704 countsByYear W20928107042023 @default.
• W2092810704 crossrefType "journal-article" @default.
• W2092810704 hasAuthorship W2092810704A5000413148 @default.
• W2092810704 hasAuthorship W2092810704A5003646625 @default.
• W2092810704 hasAuthorship W2092810704A5004835104 @default.
• W2092810704 hasAuthorship W2092810704A5011001275 @default.
• W2092810704 hasAuthorship W2092810704A5013723014 @default.
• W2092810704 hasAuthorship W2092810704A5029611751 @default.
• W2092810704 hasAuthorship W2092810704A5030663532 @default.
• W2092810704 hasAuthorship W2092810704A5036098330 @default.
• W2092810704 hasAuthorship W2092810704A5038249679 @default.
• W2092810704 hasAuthorship W2092810704A5043434841 @default.
• W2092810704 hasAuthorship W2092810704A5043496834 @default.
• W2092810704 hasAuthorship W2092810704A5049743437 @default.
• W2092810704 hasAuthorship W2092810704A5051414592 @default.
• W2092810704 hasAuthorship W2092810704A5052580641 @default.
• W2092810704 hasAuthorship W2092810704A5054977892 @default.
• W2092810704 hasAuthorship W2092810704A5057000719 @default.
• W2092810704 hasAuthorship W2092810704A5058160584 @default.
• W2092810704 hasAuthorship W2092810704A5058959179 @default.
• W2092810704 hasAuthorship W2092810704A5059055660 @default.
• W2092810704 hasAuthorship W2092810704A5069162354 @default.
• W2092810704 hasAuthorship W2092810704A5069863581 @default.
• W2092810704 hasAuthorship W2092810704A5082432143 @default.
• W2092810704 hasAuthorship W2092810704A5084426405 @default.
• W2092810704 hasAuthorship W2092810704A5091093541 @default.
• W2092810704 hasBestOaLocation W20928107042 @default.

• next