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- W2092818811 abstract "A fluorimetric detection method for one of the tryptophan metabolites, cinnabarinic acid (CA), which has recently been reported to have the ability to induce apoptosis in thymocytes, was developed using o-tolyl hydrazine (TH) as the derivatization reagent. The carbonyl group at position 3 in CA was tagged with the hydrazino moiety of TH at 100 degrees C for 30 min, and the generated derivative, CA tagged with TH, fluoresced at 412 nm with a 316 nm excitation wavelength. The CA tagged with TH was separated on a reversed-phase HPLC and detected fluorometrically. The relative standard deviation was in the range of 1.1-8.9% (n = 3), and the detection limit was approximately 12?fmol (signal-to-noise ratio, 3). The proposed HPLC method can be useful for the sensitive detection of CA." @default.
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- W2092818811 date "2009-01-01" @default.
- W2092818811 modified "2023-09-24" @default.
- W2092818811 title "Development of a fluorimetric detection method for cinnabarinic acid using<i>ortho</i>-tolyl hydrazine as the derivatization reagent" @default.
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- W2092818811 doi "https://doi.org/10.1002/bmc.1281" @default.
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