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- W2092874874 abstract "Pseudomonas sp. clone A is able to use 2,4,6-trinitrotoluene (TNT), 2,4-dinitrotoluene, and 2,6-dinitrotoluene as an N-source after the enzymatic removal of nitro groups from the aromatic ring. We have identified in culture supernatants a hydride−TNT Meisenheimer complex, which is an intermediate in the removal of the first nitro group from the TNT aromatic ring. It has been shown in vitro that the hydride−TNT Meisenheimer complex is transformed to 2,4-dinitrotoluene and an unidentified compound, the molecular mass of which is 235. As side metabolites, reduced TNT derivativeswhich cannot be used as an N-source by this bacteriumappeared in culture supernatants. 2-Hydroxylamino-4,6-dinitrotoluene, 4-hydroxylamino-2,6-dinitrotoluene, 4-amino-2,6-dinitrotoluene, 2-amino-4,6-dinitrotoluene, and 2,4-diamino-6-nitrotoluene were found and chemically characterized. Spontaneous condensation of partially reduced TNT forms leads to the production of azoxytoluenes, and a possible condensation mechanism is discussed. For novel compounds, 1H NMR, UV, and infrared data are provided. Therefore, Pseudomonas sp. clone A carries out two different initial reductive reactions in the metabolism of 2,4,6-trinitrotoluene; one process removes a nitro group from the aromatic ring and allows it to be used as an N-source, while the other gives rise to side products." @default.
- W2092874874 created "2016-06-24" @default.
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- W2092874874 date "1996-06-01" @default.
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- W2092874874 title "Identification of Products Resulting from the Biological Reduction of 2,4,6-Trinitrotoluene, 2,4-Dinitrotoluene, and 2,6-Dinitrotoluene by <i>Pseudomonas</i> sp." @default.
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- W2092874874 doi "https://doi.org/10.1021/es950824u" @default.
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