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- W2092893995 abstract "Examination of restriction site length polymorphism (RFLP) in the nuclear ribosomal RNA (rDNA) gene in four species of jungle fowl showed that a Sac I site in the external spacer region exists in the three jungle fowl, Gallus sonnerati, G. lafayettei and G. varius but is entirely absent in the domestic fowl, G. gallus. In order to understand the phylogenetic meaning, an interspecific survey on restriction site variation in 4 species of Gallus and 20 related genera that belong to 12 species of the same family Phasianidae and 8 species of the families Meleagrididae, Tetoraonidae, Odontophoridae, Numididae, Megapodidae and Cracidae that belong to the order Galliformes was performed. A Sac I fragment of 2.7kb in length was observed consistently in the members of Phasianidae, except for G. gallus, Phasianus versicolor and Lophophorus impeyanus using the Southern blot survey with the 18S mouse rDNA probe. On the other hand, the length of Sac I fragments in the other species of outgroups differed from that of the common type of Phasianidae. Our data thus indicated that the presence of the Sac I site in the spacer region was a common feature of the ancestral branch of the family Phasianidae and that the site had differentiated until it was lost in G. gallus during the course of speciation. It is suggested, therefore, that the absence of the Sac I site may be a convenient diagnostic marker of G. gallus in surveys of genetic backgrounds in stocks of domestic fowl as well as populations of jungle fowl." @default.
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- W2092893995 date "2000-01-01" @default.
- W2092893995 modified "2023-09-24" @default.
- W2092893995 title "Restriction Site Variation in Ribosomal DNA in 24 Species of the Order Galliformes" @default.
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- W2092893995 doi "https://doi.org/10.2508/chikusan.71.572" @default.
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