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- W2093089644 abstract "It is desirable to build a universal and efficient protein expression system for wild-type prokaryotic strains in biotechnology industry and the outstanding T7 expression system could be a good candidate. However, the current utilization of T7 system depends on the specific DE3 lysogenic hosts, which severely limits its application in wild-type strains. In this study, a host-independent T7 expression system without relying on DE3 lysogenic hosts to provide T7 RNA Polymerase was developed. T7 RNA Polymerase gene (Gene1) and T7 Promoter were successfully integrated into a single plasmid with the regulation of proper antisense RNA to limit T7 RNA Polymerase expression at a non-lethal level. This host-independent T7 expression system realized efficient protein expression in 4 non-DE3 Escherichia coli strains and a wild-type Sinorhizobium strain TH572." @default.
- W2093089644 created "2016-06-24" @default.
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- W2093089644 date "2014-11-01" @default.
- W2093089644 modified "2023-09-25" @default.
- W2093089644 title "Construction of a host-independent T7 expression system with small RNA regulation" @default.
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- W2093089644 doi "https://doi.org/10.1016/j.jbiotec.2014.08.039" @default.
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