FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093202361> ?p ?o ?g. }

• W2093202361 endingPage "34" @default.
• W2093202361 startingPage "34" @default.
• W2093202361 abstract "Titulo corto: Metodologia para el aislamiento e identificacion  de LPS de periodonto-patogenos Titulo en ingles: Purification and characterization of lipopolysaccharide from Eikenella corrodens 23834 and Porphyromonas gingivalis W83 Resumen:  La purificacion de lipopolisacaridos (LPS) o endotoxinas y su caracterizacion es un aspecto esencial para estudios que buscan aclarar el papel de estas biomoleculas de bacterias Gram negativas presentes en la cavidad oral y su relacion con enfermedades locales periodontales y sistemicas. Este estudio implementa una metodologia para la extraccion, purificacion y caracterizacion de LPS a partir de bacteria completa de Eikenella corrodens 23834 y Porphyromonas gingivalis W83,  utilizando tecnicas previamente descritas. La extraccion cruda de LPS se realizo con fenol-agua caliente; la purificacion se realizo con tratamiento enzimatico con nucleasas y proteasa, seguido de cromatografia de exclusion por tamano (Sephacryl S-200 HR) con deoxicolato de sodio como fase movil. La caracterizacion de los extractos purificados se realizo por barrido espectrofotometrico, pruebas bioquimicas de electroforesis SDS-PAGE, ensayo Purpald y la prueba cromogenica de LAL. Como control para la identificacion y caracterizacion de los extractos purificados se utilizaron LPS comerciales de Escherichia coli, Salmonella typhimurium, Rodobacter sphaeroides y Porphyromonas gingivalis . La metodologia implementada permitio la obtencion de LPS de elevada pureza con la identificacion de KDO o heptosas, un quimiotipo de LPS-S (liso) para E. corrodens y LPS-SR (semi-rugoso) para P. gingivalis W83 . Ambos LPS purificados mostraron capacidad endotoxica a bajas concentraciones. La metodologia implementada en este estudio para la purificacion y caracterizacion de LPS a partir de bacteria completa  fue eficiente al compararla con los LPS comerciales. Palabras clave : endotoxinas, cromatografia, acido 2-ceto-3-desoxioctulosonico (KDO), test LAL, periodontitis. Abstract:  Purification of lipopolysaccharide (LPS) or endotoxins and its characterization is an important aspect for studies aimed at clarifying the role of these biomolecules from Gram negative bacteria present in the oral cavity and its relationship with periodontal and systemic diseases. This study describes an extraction, purification and characterization method of LPS from Eikenella corrodens 23834 and Porphyromonas gingivalis W83. LPS extraction was performed using hot phenol-water; the purification was done with nuclease and protease enzymatic treatment, followed by size-exclusion chromatography (Sephacryl S-200 HR) with sodium deoxycholate as mobile phase. The characterization of the purified extracts was performed by spectrophotometric scanning, SDS-PAGE biochemical tests, Purpald assay and chromogenic LAL test. As control, commercial LPS from Escherichia coli , Salmonella typhimurium , P. gingivalis , and Rodobacter sphaeroides were used. The methodology mentioned above had allowed obtaining high purity LPS by identifying KDO or heptoses, a chemotype S-LPS (smooth) to E. corrodens ; SR-LPS (semi-rough) for P. gingivalis W83. Both purified LPS showed endotoxic capacity at low concentrations. The methodology used in this study for purification and characterization of LPS from the whole bacteria was efficient when compared with commercial LPS. Key words : endotoxin, 2-keto-3-deoxyoctonate (KDO), Chromatography, Limulus test (LAL), periodontitis." @default.
• W2093202361 created "2016-06-24" @default.
• W2093202361 creator A5019847689 @default.
• W2093202361 creator A5023538323 @default.
• W2093202361 creator A5047031930 @default.
• W2093202361 creator A5059590957 @default.
• W2093202361 creator A5059993942 @default.
• W2093202361 creator A5079874768 @default.
• W2093202361 date "2014-06-01" @default.
• W2093202361 modified "2023-09-27" @default.
• W2093202361 title "Purificación y caracterización de lipopolisacáridos de Eikenella corrodens 23834 y Porphyromonas gingivalis W83" @default.
• W2093202361 cites W1521121957 @default.
• W2093202361 cites W1535151445 @default.
• W2093202361 cites W1603509290 @default.
• W2093202361 cites W1809950776 @default.
• W2093202361 cites W1837256871 @default.
• W2093202361 cites W1922098314 @default.
• W2093202361 cites W1963500205 @default.
• W2093202361 cites W1968897935 @default.
• W2093202361 cites W1978843006 @default.
• W2093202361 cites W1985782125 @default.
• W2093202361 cites W1989896382 @default.
• W2093202361 cites W1994616586 @default.
• W2093202361 cites W1997331567 @default.
• W2093202361 cites W2000281350 @default.
• W2093202361 cites W2001839140 @default.
• W2093202361 cites W2011262069 @default.
• W2093202361 cites W2011683499 @default.
• W2093202361 cites W2020483175 @default.
• W2093202361 cites W2021752788 @default.
• W2093202361 cites W2030118873 @default.
• W2093202361 cites W2048318418 @default.
• W2093202361 cites W2064903683 @default.
• W2093202361 cites W2095060158 @default.
• W2093202361 cites W2097361915 @default.
• W2093202361 cites W2100837269 @default.
• W2093202361 cites W2102882196 @default.
• W2093202361 cites W2110873536 @default.
• W2093202361 cites W2112146497 @default.
• W2093202361 cites W2115893060 @default.
• W2093202361 cites W2122719824 @default.
• W2093202361 cites W2132663081 @default.
• W2093202361 cites W2133664523 @default.
• W2093202361 cites W2142979519 @default.
• W2093202361 cites W2143563875 @default.
• W2093202361 cites W2154339285 @default.
• W2093202361 cites W4229922201 @default.
• W2093202361 cites W4232534952 @default.
• W2093202361 cites W4234317413 @default.
• W2093202361 cites W46222886 @default.
• W2093202361 doi "https://doi.org/10.15446/rev.colomb.biote.v16n1.44224" @default.
• W2093202361 hasPublicationYear "2014" @default.
• W2093202361 type Work @default.
• W2093202361 sameAs 2093202361 @default.
• W2093202361 citedByCount "6" @default.
• W2093202361 countsByYear W20932023612017 @default.
• W2093202361 countsByYear W20932023612018 @default.
• W2093202361 countsByYear W20932023612019 @default.
• W2093202361 countsByYear W20932023612020 @default.
• W2093202361 crossrefType "journal-article" @default.
• W2093202361 hasAuthorship W2093202361A5019847689 @default.
• W2093202361 hasAuthorship W2093202361A5023538323 @default.
• W2093202361 hasAuthorship W2093202361A5047031930 @default.
• W2093202361 hasAuthorship W2093202361A5059590957 @default.
• W2093202361 hasAuthorship W2093202361A5059993942 @default.
• W2093202361 hasAuthorship W2093202361A5079874768 @default.
• W2093202361 hasBestOaLocation W20932023611 @default.
• W2093202361 hasConcept C185592680 @default.
• W2093202361 hasConcept C2778380484 @default.
• W2093202361 hasConcept C2780183776 @default.
• W2093202361 hasConcept C523546767 @default.
• W2093202361 hasConcept C54355233 @default.
• W2093202361 hasConcept C86803240 @default.
• W2093202361 hasConcept C89423630 @default.
• W2093202361 hasConceptScore W2093202361C185592680 @default.
• W2093202361 hasConceptScore W2093202361C2778380484 @default.
• W2093202361 hasConceptScore W2093202361C2780183776 @default.
• W2093202361 hasConceptScore W2093202361C523546767 @default.
• W2093202361 hasConceptScore W2093202361C54355233 @default.
• W2093202361 hasConceptScore W2093202361C86803240 @default.
• W2093202361 hasConceptScore W2093202361C89423630 @default.
• W2093202361 hasIssue "1" @default.
• W2093202361 hasLocation W20932023611 @default.
• W2093202361 hasLocation W20932023612 @default.
• W2093202361 hasLocation W20932023613 @default.
• W2093202361 hasOpenAccess W2093202361 @default.
• W2093202361 hasPrimaryLocation W20932023611 @default.
• W2093202361 hasRelatedWork W1521825266 @default.
• W2093202361 hasRelatedWork W1571511466 @default.
• W2093202361 hasRelatedWork W1594142412 @default.
• W2093202361 hasRelatedWork W1964563685 @default.
• W2093202361 hasRelatedWork W1997310856 @default.
• W2093202361 hasRelatedWork W2022756808 @default.
• W2093202361 hasRelatedWork W2076852084 @default.
• W2093202361 hasRelatedWork W2155044429 @default.
• W2093202361 hasRelatedWork W2187604711 @default.
• W2093202361 hasRelatedWork W2329996233 @default.
• W2093202361 hasVolume "16" @default.

• next