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• W2093205227 startingPage "2309" @default.
• W2093205227 abstract "The translation and degradation of mRNAs are two key steps in gene expression that are highly regulated and targeted by many factors, including microRNAs (miRNAs). While it is well established that translation and mRNA degradation are tightly coupled, it is still not entirely clear where in the cell mRNA degradation takes place. In this study, we investigated the possibility of mRNA degradation on the ribosome in Drosophila cells. Using polysome profiles and ribosome affinity purification, we could demonstrate the copurification of various deadenylation and decapping factors with ribosome complexes. Also, AGO1 and GW182, two key factors in the miRNA-mediated mRNA degradation pathway, were associated with ribosome complexes. Their copurification was dependent on intact mRNAs, suggesting the association of these factors with the mRNA rather than the ribosome itself. Furthermore, we isolated decapped mRNA degradation intermediates from ribosome complexes and performed high-throughput sequencing analysis. Interestingly, 93% of the decapped mRNA fragments (approximately 12,000) could be detected at the same relative abundance on ribosome complexes and in cell lysates. In summary, our findings strongly indicate the association of the majority of bulk mRNAs as well as mRNAs targeted by miRNAs with the ribosome during their degradation." @default.
• W2093205227 created "2016-06-24" @default.
• W2093205227 creator A5041684507 @default.
• W2093205227 creator A5042925165 @default.
• W2093205227 creator A5052103875 @default.
• W2093205227 creator A5053752556 @default.
• W2093205227 creator A5079275011 @default.
• W2093205227 date "2015-07-01" @default.
• W2093205227 modified "2023-10-16" @default.
• W2093205227 title "General and MicroRNA-Mediated mRNA Degradation Occurs on Ribosome Complexes in <i>Drosophila</i> Cells" @default.
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