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- W2093214543 abstract "The supply of taxol, a valuable anticancer compound, depends completely on the extraction of taxol from Taxus brevifolia (Pacific yew) plants. Although taxol is found in virtually all parts of the plant, nothing is known about the localization of taxol within the cells or tissues of the plant. Portions of T. cuspidata stems were chemically fixed, dehydrated in ethanol, and then embedded in standard resins. In these tissues, immunolocalization using polyclonal antitaxol antiserum indicates that taxol is associated with vacuolar tannin inclusions in axial phloem parenchyma cells. Little or no label is bound over the cell walls of any cell type. However, chemical preparation causes diffusion of taxol, which results in an erroneous localization of taxol. In contrast, using cryotechniques and a water-soluble melamine resin (Nanoplast), taxol is localized almost exclusively in the cell walls of phloem, vascular cambium, and xylem. Our method yields insight into the problems associated with the intra- and extracellular localization of lipophilic plant secondary compounds. It also offers an alternative tissue-preparation protocol that could be useful for the localization of other plant metabolites." @default.
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- W2093214543 date "1995-09-01" @default.
- W2093214543 modified "2023-09-23" @default.
- W2093214543 title "Immunocytochemical Localization of Taxol in Taxus cuspidata" @default.
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- W2093214543 doi "https://doi.org/10.1086/297289" @default.
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