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• W2093234014 abstract "Abstract An efficient optimization method for the crystallization of biological macromolecules has been developed and tested. This builds on a successful high‐throughput technique for the determination of initial crystallization conditions. The optimization method takes an initial condition identified through screening and then varies the concentration of the macromolecule, precipitant, and the growth temperature in a systematic manner. The amount of sample and number of steps is minimized and no biochemical reformulation is required. In the current application a robotic liquid handling system enables high‐throughput use, but the technique can easily be adapted in a nonautomated setting. This method has been applied successfully for the rapid optimization of crystallization conditions in nine representative cases." @default.
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• W2093234014 date "2007-04-01" @default.
• W2093234014 modified "2023-09-30" @default.
• W2093234014 title "Efficient optimization of crystallization conditions by manipulation of drop volume ratio and temperature" @default.
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• W2093234014 doi "https://doi.org/10.1110/ps.062699707" @default.
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