FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093260989> ?p ?o ?g. }

• W2093260989 abstract "The cyanobacteria Anacystis nidulans (Synechococcus sp. PCC6301), Synechocystis sp. PCC6803, Anabaena sp. PCC 7120, and Nostoc sp. PCC8009 were grown photoautotrophically under reduced oxygen tension in a medium with sulfate replaced by thiosulfate and nitrate replaced by ammonium as the S- and N-sources, respectively. In addition, Anabaena and Nostoc were grown under dinitrogen-fixing conditions in a medium free of combined nitrogen. Membranes were isolated from late-logarithmic cells (culture density corresponding to approximately 3 microliters packed cells per milliliter); cytoplasmic and thylakoid membranes were separated and purified according to established procedures. Acid-labile hemes were extracted from the membranes and subjected to reversed-phase high-performance liquid chromatography. Separated hemes were analyzed spectroscopically and identified by comparison with authentic standards. In addition to hemes B, A, and O, the latter of which was induced under semianaerobic conditions only, substitution of thiosulfate and ammonium for the oxy-anions sulfate and nitrate led to the appearance of spectrally discernible heme D in the membranes and extracts therefrom. However, spectroscopic and kinetic investigation of the membrane-bound heme D rather disproved any reaction with oxygen or carbon monoxide. Kinetic measurements performed with the membrane-bound respiratory oxidase gave evidence for only two kinetically competent terminal oxidases, a3 and o3, both apparently associated with a single type of apoprotein, viz. subunit I of the known cyanobacterial aa3-type cytochrome c oxidase. The heme D, on the other hand, seems to form a spectrally distinguished, yet kinetically ill-defined hemoprotein complex which does not qualify as a fully functional d-type terminal oxidase on our (wild-type) cyanobacteria even after growth under semianaerobic pseudo-reducing conditions. Also growth (of Anabaena and Nostoc) under dinitrogen-fixing conditions did not change this situation. Thus, we are left with (wild-type) cyanobacteria forming an unbranched respiratory chain with only a single type of terminal oxidase protein, viz. the known aa3-type cytochrome c oxidase. This oxidase, however, may incorporate different prosthetic (heme) groups in the sense of heme promiscuity. Biosynthesis of the different heme groups thereby seems to respond to the ambient redox environment. In particular, however, conditions for expression of the two quinol oxidases potentially and additionally coded for by the genome of, e. g., Synechocystis sp. PCC6803 (see http://www.kazusa.or.jp/cyano), have not yet been found." @default.
• W2093260989 created "2016-06-24" @default.
• W2093260989 creator A5002225234 @default.
• W2093260989 creator A5016388531 @default.
• W2093260989 creator A5033235422 @default.
• W2093260989 creator A5053731968 @default.
• W2093260989 creator A5082602362 @default.
• W2093260989 date "1999-07-01" @default.
• W2093260989 modified "2023-10-11" @default.
• W2093260989 title "Extended Heme Promiscuity in the Cyanobacterial Cytochrome c Oxidase: Characterization of Native Complexes Containing Hemes A, O, and D, Respectively" @default.
• W2093260989 cites W1482906021 @default.
• W2093260989 cites W1494474117 @default.
• W2093260989 cites W1534839391 @default.
• W2093260989 cites W1582893393 @default.
• W2093260989 cites W1585551835 @default.
• W2093260989 cites W1860132996 @default.
• W2093260989 cites W1869491056 @default.
• W2093260989 cites W1944966080 @default.
• W2093260989 cites W1973255798 @default.
• W2093260989 cites W1975654934 @default.
• W2093260989 cites W1986945080 @default.
• W2093260989 cites W1993487012 @default.
• W2093260989 cites W2000551612 @default.
• W2093260989 cites W2001784797 @default.
• W2093260989 cites W2007451793 @default.
• W2093260989 cites W2007709095 @default.
• W2093260989 cites W2016214213 @default.
• W2093260989 cites W2026694535 @default.
• W2093260989 cites W2028290643 @default.
• W2093260989 cites W2033555996 @default.
• W2093260989 cites W2036444772 @default.
• W2093260989 cites W2042174473 @default.
• W2093260989 cites W2043128060 @default.
• W2093260989 cites W2050002964 @default.
• W2093260989 cites W2057864875 @default.
• W2093260989 cites W2072001403 @default.
• W2093260989 cites W2088283939 @default.
• W2093260989 cites W2118325780 @default.
• W2093260989 cites W2155006843 @default.
• W2093260989 cites W2416996723 @default.
• W2093260989 cites W4295127969 @default.
• W2093260989 doi "https://doi.org/10.1006/abbi.1999.1236" @default.
• W2093260989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10375407" @default.
• W2093260989 hasPublicationYear "1999" @default.
• W2093260989 type Work @default.
• W2093260989 sameAs 2093260989 @default.
• W2093260989 citedByCount "14" @default.
• W2093260989 countsByYear W20932609892014 @default.
• W2093260989 countsByYear W20932609892016 @default.
• W2093260989 crossrefType "journal-article" @default.
• W2093260989 hasAuthorship W2093260989A5002225234 @default.
• W2093260989 hasAuthorship W2093260989A5016388531 @default.
• W2093260989 hasAuthorship W2093260989A5033235422 @default.
• W2093260989 hasAuthorship W2093260989A5053731968 @default.
• W2093260989 hasAuthorship W2093260989A5082602362 @default.
• W2093260989 hasConcept C101692577 @default.
• W2093260989 hasConcept C104317684 @default.
• W2093260989 hasConcept C123249941 @default.
• W2093260989 hasConcept C141280058 @default.
• W2093260989 hasConcept C178790620 @default.
• W2093260989 hasConcept C181199279 @default.
• W2093260989 hasConcept C181440489 @default.
• W2093260989 hasConcept C185592680 @default.
• W2093260989 hasConcept C20705724 @default.
• W2093260989 hasConcept C2776217839 @default.
• W2093260989 hasConcept C2777530773 @default.
• W2093260989 hasConcept C2779669040 @default.
• W2093260989 hasConcept C2780265247 @default.
• W2093260989 hasConcept C2780768313 @default.
• W2093260989 hasConcept C38485361 @default.
• W2093260989 hasConcept C523546767 @default.
• W2093260989 hasConcept C537208039 @default.
• W2093260989 hasConcept C54355233 @default.
• W2093260989 hasConcept C55493867 @default.
• W2093260989 hasConcept C69305403 @default.
• W2093260989 hasConcept C71240020 @default.
• W2093260989 hasConcept C86803240 @default.
• W2093260989 hasConceptScore W2093260989C101692577 @default.
• W2093260989 hasConceptScore W2093260989C104317684 @default.
• W2093260989 hasConceptScore W2093260989C123249941 @default.
• W2093260989 hasConceptScore W2093260989C141280058 @default.
• W2093260989 hasConceptScore W2093260989C178790620 @default.
• W2093260989 hasConceptScore W2093260989C181199279 @default.
• W2093260989 hasConceptScore W2093260989C181440489 @default.
• W2093260989 hasConceptScore W2093260989C185592680 @default.
• W2093260989 hasConceptScore W2093260989C20705724 @default.
• W2093260989 hasConceptScore W2093260989C2776217839 @default.
• W2093260989 hasConceptScore W2093260989C2777530773 @default.
• W2093260989 hasConceptScore W2093260989C2779669040 @default.
• W2093260989 hasConceptScore W2093260989C2780265247 @default.
• W2093260989 hasConceptScore W2093260989C2780768313 @default.
• W2093260989 hasConceptScore W2093260989C38485361 @default.
• W2093260989 hasConceptScore W2093260989C523546767 @default.
• W2093260989 hasConceptScore W2093260989C537208039 @default.
• W2093260989 hasConceptScore W2093260989C54355233 @default.
• W2093260989 hasConceptScore W2093260989C55493867 @default.
• W2093260989 hasConceptScore W2093260989C69305403 @default.
• W2093260989 hasConceptScore W2093260989C71240020 @default.
• W2093260989 hasConceptScore W2093260989C86803240 @default.
• W2093260989 hasLocation W20932609891 @default.

• next