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- W209326535 abstract "A novel method for the preparation of intact chromatin from the slime mold Physarumpolycephalum> which retains the invivo property of RNA synthesis is described. Preparations from G2-cells were highly active, while those from metaphase-cells were inactive. The plasmodial cells were disrupted by gentle homogenization on a polyethylene sieve in a neutral isotonic sucrose medium containing Mg++, deoxycholate and EGTA, a Ca++-chelating agent. The nuclei were lysed in a hypotonic buffer without use of EDTA and chromatin was precipitated by centrifugation after addition of Mg++." @default.
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- W209326535 date "1982-01-01" @default.
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- W209326535 title "Preparation of metaphase chromatin of Physarumpolycephalum without the loss of repressed RNA synthesis" @default.
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- W209326535 doi "https://doi.org/10.1016/0006-291x(82)90687-8" @default.
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