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- W2093385518 abstract "The nolK gene of Azorhizobium caulinodans is essential for the incorporation of a fucosyl group in Nod factors. A NAD(P)‐binding site is present in the NolK amino acid sequence and the gene is homologous to Escherichia coli genes, presumably involved in GDP‐fucose synthesis. Protein extracts of A. caulinodans , overexpressing nolK , have an enzyme activity that synthesizes GDP‐fucose from GDP‐mannose. nolK most probably encodes a 4‐reductase performing the last step in GDP‐fucose synthesis. Wild‐type A. caulinodans produces a population of fucosylated and non‐fucosylated molecules but the nolK ‐overexpressing strain produces only fucosylated Nod factors. Thus, the production of activated fucosyl donors is a rate‐limiting step in Nod factor fucosylation." @default.
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- W2093385518 date "1997-06-09" @default.
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- W2093385518 title "The nodulation gene<i>nolK</i>of<i>Azorhizobium caulinodans</i>is involved in the formation of GDP-fucose from GDP-mannose" @default.
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- W2093385518 doi "https://doi.org/10.1016/s0014-5793(97)00461-4" @default.
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