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• W2093417927 endingPage "015002" @default.
• W2093417927 startingPage "015002" @default.
• W2093417927 abstract "Two-dimensional (2D) culture systems provide useful information about many biological processes. However, some applications including tissue engineering, drug transport studies, and analysis of cell growth and dynamics are better studied using three-dimensional (3D) culture systems. 3D culture systems can potentially offer higher degrees of organization and control of cell growth environments, more physiologically relevant diffusion characteristics, and permit the formation of more extensive 3D networks of cell–cell interactions. A 3D culture system has been developed using alginate as a cell scaffold, capable of maintaining the viability and function of a variety of neural cell types. Alginate was functionalized by the covalent attachment of a variety of whole proteins and peptide epitopes selected to provide sites for cell attachment. Alginate constructs were used to entrap a variety of neural cell types including astroglioma cells, astrocytes, microglia and neurons. Neural cells displayed process outgrowth over time in culture. Cell-seeded scaffolds were characterized in terms of their biochemical and biomechanical properties, effects on seeded neural cells, and suitability for use as 3D neural cell culture models." @default.
• W2093417927 created "2016-06-24" @default.
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• W2093417927 date "2011-01-05" @default.
• W2093417927 modified "2023-10-12" @default.
• W2093417927 title "Fabrication and optimization of alginate hydrogel constructs for use in 3D neural cell culture" @default.
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• W2093417927 doi "https://doi.org/10.1088/1748-6041/6/1/015002" @default.
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