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• W2093485304 endingPage "1419" @default.
• W2093485304 startingPage "1403" @default.
• W2093485304 abstract "Digital, video-enhanced fluorescence microscopy and spectrofluorometry were used to follow the internalization into the yeast Saccharomyces cerevisiae of phosphatidylcholine molecules labeled on one acyl chain with the fluorescent probe 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD). Two pathways were found: (1) transport by endocytosis to the vacuole and (2) transport by a non-endocytic pathway to the nuclear envelope and mitochondria. The endocytic pathway was inhibited at low temperature (< 2 degrees C) and by ATP depletion. Mutations in secretory (SEC) genes that are necessary for membrane traffic through the secretory pathway (including SEC1, SEC2, SEC4, SEC6, SEC7, SEC12, SEC14, SEC17, SEC18, and SEC21) almost completely blocked endocytic uptake. In contrast, mutations in the SEC63, SEC65, or SEC11 genes, required for translocation of nascent secretory polypeptides into the ER or signal peptide processing in the ER, only slightly reduced endocytic uptake. Phospholipid endocytosis was also independent of the gene encoding the clathrin heavy chain, CHC1. The correlation of biochemical analysis with fluorescence microscopy indicated that the fluorescent phosphatidylcholine was degraded in the vacuole and that degradation was, at least in part, dependent on the vacuolar proteolytic cascade. The non-endocytic route functioned with a lower cellular energy charge (ATP levels 80% reduced) and was largely independent of the SEC genes. Non-endocytic transport of NBD-phosphatidylcholine to the nuclear envelope and mitochondria was inhibited by pretreatment of cells with the sulfhydryl reagents N-ethylmaleimide and p-chloromercuribenzenesulfonic acid, suggesting the existence of protein-mediated transmembrane transfer (flip-flop) of phosphatidylcholine across the yeast plasma membrane. These data establish a link between lipid movement during secretion and endocytosis in yeast and suggest that phospholipids may also gain access to intracellular organelles through non-endocytic, protein-mediated events." @default.
• W2093485304 created "2016-06-24" @default.
• W2093485304 creator A5027478389 @default.
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• W2093485304 date "1993-12-15" @default.
• W2093485304 modified "2023-09-26" @default.
• W2093485304 title "Retrograde lipid traffic in yeast: identification of two distinct pathways for internalization of fluorescent-labeled phosphatidylcholine from the plasma membrane." @default.
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• W2093485304 cites W1527093201 @default.
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• W2093485304 cites W1561840692 @default.
• W2093485304 cites W1568987948 @default.
• W2093485304 cites W1573954448 @default.
• W2093485304 cites W1578213988 @default.
• W2093485304 cites W1596493745 @default.
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• W2093485304 cites W1988420633 @default.
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• W2093485304 cites W2009421164 @default.
• W2093485304 cites W2014826463 @default.
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• W2093485304 cites W2022936503 @default.
• W2093485304 cites W2033980199 @default.
• W2093485304 cites W2039526503 @default.
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• W2093485304 cites W2079588007 @default.
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• W2093485304 doi "https://doi.org/10.1083/jcb.123.6.1403" @default.
• W2093485304 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2290883" @default.
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