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• W2093500876 abstract "Potassium channel blockers have been shown to possess antitumor properties, but the role of apoptosis remains to be clarified. In this study, we investigated the antiproliferative effect of tetraethylammonium (TEA), a nonspecific potassium channel blocker, in rat C6 and 9L glioma cells.Cytotoxicity was evaluated by MTT assay. Apoptosis was detected by TUNEL and annexin V-FITC/propidium iodide assays. Protein levels were determined by Western blot analysis. Intracellular reactive oxygen species (ROS) levels were assessed flow cytometrically.TEA (2-60 mM) significantly inhibited the proliferation of C6 and 9L glioma cells. In addition, increased cell apoptosis was confirmed after treatment with 40 mM TEA. Apoptosis was associated with a dramatic increase in ROS levels and altered Bcl-2/Bax protein balance.TEA can inhibit proliferation and induce apoptosis in both cell lines; therefore, it might be associated with the increase in intracellular ROS production." @default.
• W2093500876 created "2016-06-24" @default.
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• W2093500876 date "2009-01-01" @default.
• W2093500876 modified "2023-10-18" @default.
• W2093500876 title "Tetraethylammonium Inhibits Glioma Cells via Increasing Production of Intracellular Reactive Oxygen Species" @default.
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• W2093500876 doi "https://doi.org/10.1159/000235730" @default.
• W2093500876 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19707016" @default.
• W2093500876 hasPublicationYear "2009" @default.
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