FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093576331> ?p ?o ?g. }

• W2093576331 endingPage "307" @default.
• W2093576331 startingPage "305" @default.
• W2093576331 abstract "Phenylalanine hydroxylase (PAH) is a non-heme iron dioxygenase catalyzing the conversion of phenylalanine to tyrosine and is present in both prokaryotic and eukaryotic organisms. A relatively simple PAH is expressed by Chromobacterium violaceum, a gram-negative bacterium found in tropical and subtropical regions. The effects of temperature, pH and metals on the stability and catalytic activity of Chromobacterium violaceum PAH were determined by steady-state kinetics, circular dichroism (CD) and differential scanning calorimetry (DSC). The kcat and KM for phenylalanine were determined between 7 and 40 °C. The KM remained constant between 20 and 40 °C but rapidly increased below 20 °C. The half-life of the enzyme at 47 °C is 66 ± 4 min in the presence of Fe(II) and 8 ± 1 min in the presence of EDTA. The melting temperature of the protein determined by CD and DSC is 53 ± 2 °C in the presence of EDTA and 63 ± 2 °C in the presence of Fe(II). Co(II) stabilizes the enzyme (Tm = 63 ± 2 °C) and inhibits the catalytic activity by displacing iron from the active site. The optimum pH for catalytic activity and stability is 7.4. In conclusion, PAH is adapted for optimal phenylalanine binding at temperatures above 20 °C and Fe(II) enhances the resistance of the enzyme to thermal denaturation." @default.
• W2093576331 created "2016-06-24" @default.
• W2093576331 creator A5047990170 @default.
• W2093576331 creator A5053442721 @default.
• W2093576331 creator A5088228988 @default.
• W2093576331 date "2002-12-01" @default.
• W2093576331 modified "2023-10-16" @default.
• W2093576331 title "Use of a tyrosine hydroxylase mutant enzyme with reduced metal affinity allows detection of activity with cobalt in place of iron" @default.
• W2093576331 cites W1986049046 @default.
• W2093576331 cites W1993409207 @default.
• W2093576331 cites W2004226471 @default.
• W2093576331 cites W2040840111 @default.
• W2093576331 cites W2046036275 @default.
• W2093576331 cites W2052844412 @default.
• W2093576331 cites W2075959103 @default.
• W2093576331 cites W2076727938 @default.
• W2093576331 cites W2086172007 @default.
• W2093576331 cites W2087967880 @default.
• W2093576331 cites W2089317626 @default.
• W2093576331 cites W2100176912 @default.
• W2093576331 cites W2115297948 @default.
• W2093576331 cites W2953079496 @default.
• W2093576331 doi "https://doi.org/10.1016/s0003-9861(02)00568-4" @default.
• W2093576331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12464285" @default.
• W2093576331 hasPublicationYear "2002" @default.
• W2093576331 type Work @default.
• W2093576331 sameAs 2093576331 @default.
• W2093576331 citedByCount "4" @default.
• W2093576331 countsByYear W20935763312016 @default.
• W2093576331 crossrefType "journal-article" @default.
• W2093576331 hasAuthorship W2093576331A5047990170 @default.
• W2093576331 hasAuthorship W2093576331A5053442721 @default.
• W2093576331 hasAuthorship W2093576331A5088228988 @default.
• W2093576331 hasConcept C104317684 @default.
• W2093576331 hasConcept C118687296 @default.
• W2093576331 hasConcept C121332964 @default.
• W2093576331 hasConcept C133571119 @default.
• W2093576331 hasConcept C13965031 @default.
• W2093576331 hasConcept C148898269 @default.
• W2093576331 hasConcept C161790260 @default.
• W2093576331 hasConcept C179104552 @default.
• W2093576331 hasConcept C181199279 @default.
• W2093576331 hasConcept C185592680 @default.
• W2093576331 hasConcept C207009774 @default.
• W2093576331 hasConcept C2776165026 @default.
• W2093576331 hasConcept C2777431362 @default.
• W2093576331 hasConcept C2777916119 @default.
• W2093576331 hasConcept C39519442 @default.
• W2093576331 hasConcept C41183919 @default.
• W2093576331 hasConcept C515207424 @default.
• W2093576331 hasConcept C515602321 @default.
• W2093576331 hasConcept C55493867 @default.
• W2093576331 hasConcept C56856141 @default.
• W2093576331 hasConcept C60987743 @default.
• W2093576331 hasConcept C62520636 @default.
• W2093576331 hasConcept C71240020 @default.
• W2093576331 hasConcept C97355855 @default.
• W2093576331 hasConceptScore W2093576331C104317684 @default.
• W2093576331 hasConceptScore W2093576331C118687296 @default.
• W2093576331 hasConceptScore W2093576331C121332964 @default.
• W2093576331 hasConceptScore W2093576331C133571119 @default.
• W2093576331 hasConceptScore W2093576331C13965031 @default.
• W2093576331 hasConceptScore W2093576331C148898269 @default.
• W2093576331 hasConceptScore W2093576331C161790260 @default.
• W2093576331 hasConceptScore W2093576331C179104552 @default.
• W2093576331 hasConceptScore W2093576331C181199279 @default.
• W2093576331 hasConceptScore W2093576331C185592680 @default.
• W2093576331 hasConceptScore W2093576331C207009774 @default.
• W2093576331 hasConceptScore W2093576331C2776165026 @default.
• W2093576331 hasConceptScore W2093576331C2777431362 @default.
• W2093576331 hasConceptScore W2093576331C2777916119 @default.
• W2093576331 hasConceptScore W2093576331C39519442 @default.
• W2093576331 hasConceptScore W2093576331C41183919 @default.
• W2093576331 hasConceptScore W2093576331C515207424 @default.
• W2093576331 hasConceptScore W2093576331C515602321 @default.
• W2093576331 hasConceptScore W2093576331C55493867 @default.
• W2093576331 hasConceptScore W2093576331C56856141 @default.
• W2093576331 hasConceptScore W2093576331C60987743 @default.
• W2093576331 hasConceptScore W2093576331C62520636 @default.
• W2093576331 hasConceptScore W2093576331C71240020 @default.
• W2093576331 hasConceptScore W2093576331C97355855 @default.
• W2093576331 hasIssue "2" @default.
• W2093576331 hasLocation W20935763311 @default.
• W2093576331 hasLocation W20935763312 @default.
• W2093576331 hasOpenAccess W2093576331 @default.
• W2093576331 hasPrimaryLocation W20935763311 @default.
• W2093576331 hasRelatedWork W1418014437 @default.
• W2093576331 hasRelatedWork W1451322921 @default.
• W2093576331 hasRelatedWork W1981144665 @default.
• W2093576331 hasRelatedWork W2019192133 @default.
• W2093576331 hasRelatedWork W2023953960 @default.
• W2093576331 hasRelatedWork W2052844412 @default.
• W2093576331 hasRelatedWork W2055622823 @default.
• W2093576331 hasRelatedWork W2061911647 @default.
• W2093576331 hasRelatedWork W2071379164 @default.
• W2093576331 hasRelatedWork W2808318619 @default.
• W2093576331 hasVolume "408" @default.
• W2093576331 isParatext "false" @default.

• next