FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093577783> ?p ?o ?g. }

• W2093577783 endingPage "220" @default.
• W2093577783 startingPage "209" @default.
• W2093577783 abstract "The ability of the ocular surface to respond to pathogens is in part attributed to toll-like receptors (TLRs) that recognize conserved motifs on various microbes. This study examines TLR expression on various ocular surface cells, if TLR agonists can modulate the expression of antimicrobial peptides (AMPs), human beta defensins (hBD) and cathelicidin (hCAP-18/LL-37) which maybe functionally active against Pseudomonas aeruginosa (PA) and if TLR agonists or AMPs can modulate TLR mRNA expression. TLR1-10 mRNA expression was examined in corneal epithelial, corneal stromal cells and conjunctival epithelial cells by RT-PCR. To confirm protein expression flow cytometry or immunostaining was performed for selected TLRs on some cell cultures. Ocular surface cells were cultured with a range of TLR agonists and then hBD-1, 2, 3, or hCAP-18 mRNA and protein expression was determined by RT-PCR and immunoblotting. In some experiments, cells were cultured with a cocktail of agonists for TLR3, 5 and 6/2 and the antimicrobial activity of the culture media was tested against PA. TLR mRNA expression was also examined in primary human corneal epithelial cells (HCEC) treated with either 3 μg/ml of hBD-2, 5 μg/ml of LL-37 or TLR4, 5 and 9 agonists. Overall, the ocular surface cells expressed mRNA for most of the TLRs but some differences were found. TLR2 was not detected in corneal fibroblasts, TLR4 was not detected in primary cultured or freshly isolated HCEC, TLR5 was not detected in conjunctival epithelial cells (IOBA-NHC) and corneal fibroblasts, TLR7 was not detected in freshly isolated HCEC and TLR10 was not detected in HCEC and IOBA-NHC. TLR8 mRNA was not expressed by any of the samples tested. Immunostaining of cadaver corneas revealed TLR5 and 9 expression throughout the cornea while TLR3 was significantly expressed only in the epithelium. Flow cytometry and immunostaining revealed cultured fibroblasts expressed TLR9 but had no significant TLR3 expression. hBD-2 expression was upregulated by TLR1/2, 3, 4, 5 and 6/2 agonists depending on the cell type, whereas only the TLR3 agonist upregulated the expression of hCAP-18 in primary HCEC. The combination of TLR3, 5 and 6/2 agonists in primary HCEC, upregulated hBD-2 and hCAP-18 mRNA and peptide expression and secretion into the culture media, which significantly killed PA. This antimicrobial activity was primarily attributed to LL-37. TLR agonists did not modulate TLR expression itself, however, LL-37 or hBD-2 downregulated TLR5, 7 and/or 9 mRNA depending on the cell type. TLRs are expressed on the ocular surface and TLR agonists trigger the production of LL-37 and hBD-2, with LL-37 being particularly important for protecting the ocular surface against PA infection." @default.
• W2093577783 created "2016-06-24" @default.
• W2093577783 creator A5066011023 @default.
• W2093577783 creator A5066617821 @default.
• W2093577783 creator A5084515179 @default.
• W2093577783 date "2011-03-01" @default.
• W2093577783 modified "2023-09-30" @default.
• W2093577783 title "Toll-like receptor activation modulates antimicrobial peptide expression by ocular surface cells" @default.
• W2093577783 cites W1514540900 @default.
• W2093577783 cites W1519633296 @default.
• W2093577783 cites W1536952772 @default.
• W2093577783 cites W1564532471 @default.
• W2093577783 cites W1580698661 @default.
• W2093577783 cites W1602448053 @default.
• W2093577783 cites W1863323524 @default.
• W2093577783 cites W1875764348 @default.
• W2093577783 cites W1917677571 @default.
• W2093577783 cites W1932041686 @default.
• W2093577783 cites W1966402088 @default.
• W2093577783 cites W1966552455 @default.
• W2093577783 cites W1968931227 @default.
• W2093577783 cites W1969072247 @default.
• W2093577783 cites W1971670144 @default.
• W2093577783 cites W1977706363 @default.
• W2093577783 cites W1981465837 @default.
• W2093577783 cites W1983066769 @default.
• W2093577783 cites W1983174976 @default.
• W2093577783 cites W1990834447 @default.
• W2093577783 cites W1992153927 @default.
• W2093577783 cites W1994391392 @default.
• W2093577783 cites W1995519698 @default.
• W2093577783 cites W2002565061 @default.
• W2093577783 cites W2002605819 @default.
• W2093577783 cites W2003738186 @default.
• W2093577783 cites W2013176877 @default.
• W2093577783 cites W2021193191 @default.
• W2093577783 cites W2022947195 @default.
• W2093577783 cites W2023306690 @default.
• W2093577783 cites W2023494896 @default.
• W2093577783 cites W2025765618 @default.
• W2093577783 cites W2025813789 @default.
• W2093577783 cites W2026251111 @default.
• W2093577783 cites W2030611349 @default.
• W2093577783 cites W2043771202 @default.
• W2093577783 cites W2047250566 @default.
• W2093577783 cites W2048389635 @default.
• W2093577783 cites W2050478892 @default.
• W2093577783 cites W2054602599 @default.
• W2093577783 cites W2055856930 @default.
• W2093577783 cites W2065361083 @default.
• W2093577783 cites W2065876844 @default.
• W2093577783 cites W2072896974 @default.
• W2093577783 cites W2076361525 @default.
• W2093577783 cites W2085269931 @default.
• W2093577783 cites W2092685383 @default.
• W2093577783 cites W2095725397 @default.
• W2093577783 cites W2099482588 @default.
• W2093577783 cites W2104159258 @default.
• W2093577783 cites W2105575479 @default.
• W2093577783 cites W2108766385 @default.
• W2093577783 cites W2113769407 @default.
• W2093577783 cites W2115360262 @default.
• W2093577783 cites W2120729063 @default.
• W2093577783 cites W2121596041 @default.
• W2093577783 cites W2125935614 @default.
• W2093577783 cites W2126734687 @default.
• W2093577783 cites W2126829776 @default.
• W2093577783 cites W2131158736 @default.
• W2093577783 cites W2133449490 @default.
• W2093577783 cites W2138363231 @default.
• W2093577783 cites W2138811813 @default.
• W2093577783 cites W2148579227 @default.
• W2093577783 cites W2151777859 @default.
• W2093577783 cites W2153328681 @default.
• W2093577783 cites W2153461044 @default.
• W2093577783 cites W2164128280 @default.
• W2093577783 cites W2168695476 @default.
• W2093577783 cites W2170021909 @default.
• W2093577783 cites W280020874 @default.
• W2093577783 cites W4229662491 @default.
• W2093577783 doi "https://doi.org/10.1016/j.exer.2010.12.005" @default.
• W2093577783 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3049847" @default.
• W2093577783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21195713" @default.
• W2093577783 hasPublicationYear "2011" @default.
• W2093577783 type Work @default.
• W2093577783 sameAs 2093577783 @default.
• W2093577783 citedByCount "79" @default.
• W2093577783 countsByYear W20935777832012 @default.
• W2093577783 countsByYear W20935777832013 @default.
• W2093577783 countsByYear W20935777832014 @default.
• W2093577783 countsByYear W20935777832015 @default.
• W2093577783 countsByYear W20935777832016 @default.
• W2093577783 countsByYear W20935777832017 @default.
• W2093577783 countsByYear W20935777832018 @default.
• W2093577783 countsByYear W20935777832019 @default.
• W2093577783 countsByYear W20935777832020 @default.
• W2093577783 countsByYear W20935777832021 @default.
• W2093577783 countsByYear W20935777832022 @default.

• next