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- W2093650657 abstract "Flow-injection spectrophotometric analysis has been established for the determination of zinc at 1 μg ml−1 level in serum using 5-Br-PAPS (2-(5-bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol) disodium salt) as a chromo-genic reagent. A 100 μl portion of serum is directly injected into the carrier stream containing 5%(v/v) TMAH (tetramethylammonium hydroxide) and 0.5 M trisodium citrate. After mixing with 0.01%(w/v) 5-Br-PAPS-0.5 M ammonium acetate-7.2×10−3 M salicylaldehyde oxime-1%(v/v) Triton X-100-0.5 M hydrochloric acid solution, the absorbance of Zn-5-Br-PAPS complex is measured at 560 nm. The proposed flow-injection system is fully controlled with a personal computer and permits 60 samples to be measured per hour. The relative standard deviation (n=50) for 1.2 μgZn mh−1 solution is 3.3%." @default.
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- W2093650657 date "1994-10-01" @default.
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- W2093650657 title "Direct Determination of Zinc in Human Serum by Flow-Injection Spectrophotometric Analysis" @default.
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