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- W2093665296 abstract "Synthesis of cysteinyl leukotrienes was assessed in patients with atopic dermatitis (AD; n=8) and healthy volunteers (n=8) by measuring urinary excretion of leukotriene E4 (LTE4), the main index metabolite of cysteinyl leukotrienes in man. Using this non‐invasive method we demonstrated a significant (P<0.05) 4.5‐fold increase in excretion of LTE4 compared with healthy volunteers. The identity of LTE4 was unequivocally demonstrated by gas chromatography‐mass spectrometry/mass spectrometry (GC‐MS/MS). LTE4 was routinely measured by radioimmunoassay (RIA), and quantitative measurement of LTE4 by RIA was validated by GC‐MS/MS. There was a linear correlation between LTE4 measured by RIA and by GC‐MS/MS (r=0.994). Tn representative samples, LTE4 was also quantitatively assessed by GC‐MS/MS. In these samples, LTE4 values obtained by GC‐MS/MS differed <10% from those obtained by RIA. The present findings suggest that cysteinyl leukotrienes play a role in AD." @default.
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- W2093665296 date "1993-06-01" @default.
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- W2093665296 title "Enhanced synthesis of cysteinyl leukotrienes in atopic dermatitis" @default.
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- W2093665296 doi "https://doi.org/10.1111/j.1365-2133.1993.tb00256.x" @default.
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