Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093669218> ?p ?o ?g. }
- W2093669218 endingPage "1264" @default.
- W2093669218 startingPage "1256" @default.
- W2093669218 abstract "Aims: The objective of the present study was to determine the potential of promoter sequences from the cfp gene of Neurospora crassa to drive the expression of transgenes in filamentous fungi. Methods and Results: Northern blot analyses showed that the mRNA levels of cfp were rapidly modified in response to either inducing or repressing culture conditions. The hygromycin phosphotransferase (hph) and S-adenosylmethionine synthetase (eth-1) genes were fused to a minimal cfp promoter fragment (Pcfp) and used as reporter genes. These constructs were highly expressed in transformant N. crassa strains grown in media containing glucose or sucrose and repressed in media containing ethanol or ethanol plus glucose. A gene fusion of the cfp promoter to the β-glucuronidase gene (cfp-uidA) showed identical patterns of expression in the heterologous filamentous fungus Aspergillus nidulans. Conclusions: Our results show that the levels of expression of the native cfp gene, as well as reporter genes driven by cfp promoter sequences, can be rapidly modified in response to different carbon sources. These modified levels of expression are maintained by continuous growth in the presence of the corresponding carbon source. Significance and Impact of the Study: We propose that the cfp promoter can be used to control the expression of transgenes in filamentous fungi in a carbon source-dependent fashion." @default.
- W2093669218 created "2016-06-24" @default.
- W2093669218 creator A5030458091 @default.
- W2093669218 creator A5033822000 @default.
- W2093669218 creator A5039071726 @default.
- W2093669218 creator A5049256320 @default.
- W2093669218 creator A5087562837 @default.
- W2093669218 date "2004-06-01" @default.
- W2093669218 modified "2023-10-06" @default.
- W2093669218 title "The Neurospora crassa cfp promoter drives a carbon source-dependent expression of transgenes in filamentous fungi" @default.
- W2093669218 cites W1555086548 @default.
- W2093669218 cites W1607006528 @default.
- W2093669218 cites W1946135113 @default.
- W2093669218 cites W1964205809 @default.
- W2093669218 cites W1978862919 @default.
- W2093669218 cites W1986475424 @default.
- W2093669218 cites W2009600767 @default.
- W2093669218 cites W2019398589 @default.
- W2093669218 cites W2027312376 @default.
- W2093669218 cites W2036631423 @default.
- W2093669218 cites W2039012340 @default.
- W2093669218 cites W2041602553 @default.
- W2093669218 cites W2041940725 @default.
- W2093669218 cites W2047234711 @default.
- W2093669218 cites W2047554740 @default.
- W2093669218 cites W2047798770 @default.
- W2093669218 cites W2049113731 @default.
- W2093669218 cites W2049286082 @default.
- W2093669218 cites W2050225134 @default.
- W2093669218 cites W2058256157 @default.
- W2093669218 cites W2077742670 @default.
- W2093669218 cites W2079435054 @default.
- W2093669218 cites W2085529614 @default.
- W2093669218 cites W2093006590 @default.
- W2093669218 cites W2095915583 @default.
- W2093669218 cites W2115319973 @default.
- W2093669218 cites W2121284595 @default.
- W2093669218 cites W2128244906 @default.
- W2093669218 cites W2139583528 @default.
- W2093669218 cites W2145543625 @default.
- W2093669218 cites W2152161315 @default.
- W2093669218 cites W2155320896 @default.
- W2093669218 cites W2246455958 @default.
- W2093669218 cites W2342421323 @default.
- W2093669218 cites W2592083376 @default.
- W2093669218 cites W2592997603 @default.
- W2093669218 cites W4213265720 @default.
- W2093669218 cites W4239967169 @default.
- W2093669218 cites W4251703648 @default.
- W2093669218 doi "https://doi.org/10.1111/j.1365-2672.2004.02249.x" @default.
- W2093669218 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15139917" @default.
- W2093669218 hasPublicationYear "2004" @default.
- W2093669218 type Work @default.
- W2093669218 sameAs 2093669218 @default.
- W2093669218 citedByCount "14" @default.
- W2093669218 countsByYear W20936692182014 @default.
- W2093669218 countsByYear W20936692182015 @default.
- W2093669218 countsByYear W20936692182016 @default.
- W2093669218 countsByYear W20936692182019 @default.
- W2093669218 countsByYear W20936692182020 @default.
- W2093669218 countsByYear W20936692182022 @default.
- W2093669218 countsByYear W20936692182023 @default.
- W2093669218 crossrefType "journal-article" @default.
- W2093669218 hasAuthorship W2093669218A5030458091 @default.
- W2093669218 hasAuthorship W2093669218A5033822000 @default.
- W2093669218 hasAuthorship W2093669218A5039071726 @default.
- W2093669218 hasAuthorship W2093669218A5049256320 @default.
- W2093669218 hasAuthorship W2093669218A5087562837 @default.
- W2093669218 hasConcept C101762097 @default.
- W2093669218 hasConcept C102230213 @default.
- W2093669218 hasConcept C104317684 @default.
- W2093669218 hasConcept C108305142 @default.
- W2093669218 hasConcept C143065580 @default.
- W2093669218 hasConcept C150194340 @default.
- W2093669218 hasConcept C153911025 @default.
- W2093669218 hasConcept C161733203 @default.
- W2093669218 hasConcept C2776739539 @default.
- W2093669218 hasConcept C2776923806 @default.
- W2093669218 hasConcept C2777251434 @default.
- W2093669218 hasConcept C40767141 @default.
- W2093669218 hasConcept C55493867 @default.
- W2093669218 hasConcept C86803240 @default.
- W2093669218 hasConceptScore W2093669218C101762097 @default.
- W2093669218 hasConceptScore W2093669218C102230213 @default.
- W2093669218 hasConceptScore W2093669218C104317684 @default.
- W2093669218 hasConceptScore W2093669218C108305142 @default.
- W2093669218 hasConceptScore W2093669218C143065580 @default.
- W2093669218 hasConceptScore W2093669218C150194340 @default.
- W2093669218 hasConceptScore W2093669218C153911025 @default.
- W2093669218 hasConceptScore W2093669218C161733203 @default.
- W2093669218 hasConceptScore W2093669218C2776739539 @default.
- W2093669218 hasConceptScore W2093669218C2776923806 @default.
- W2093669218 hasConceptScore W2093669218C2777251434 @default.
- W2093669218 hasConceptScore W2093669218C40767141 @default.
- W2093669218 hasConceptScore W2093669218C55493867 @default.
- W2093669218 hasConceptScore W2093669218C86803240 @default.
- W2093669218 hasIssue "6" @default.
- W2093669218 hasLocation W20936692181 @default.