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- W2093669812 abstract "Eighty-eight temperature-sensitive (ts) mutants of adenovirus 12, which did not replicate at 38° but did replicate well at 31° were isolated. Thirty-four of these mutants were selected and classified into 13 groups (groups A to M) by complementation tests. Analyses of the defects in viral replication at the nonpermissive temperature (38°) revealed that mutants in 4 groups (groups A, B, C, and D) were defective in the production of hexon, fiber, and penton base, mutants in group E were defective in the production of both fiber and penton base, mutants in group F were defective in the production of both hexon and penton base, a mutant in group G was defective in the production of both hexon and fiber, mutants in group H were defective in the production of only hexon, mutants in group I were defective in the production of only fiber, mutants in group J were defective in the production of only penton base and mutants in 3 groups (groups K, L, and M) were not defective in the synthesis of hexon, fiber, and penton base. These defectiveness were detected by serological reaction and polyacrylamide gel electrophoresis. Immunofluorescent examinations of cells infected with these mutants as well as the examination of heat stability of virions produced at the permissive temperature showed differences among these groups." @default.
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- W2093669812 date "1972-07-01" @default.
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- W2093669812 title "Isolation and a preliminary characterization of temperature-sensitive mutants of adenovirus 12" @default.
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- W2093669812 doi "https://doi.org/10.1016/s0042-6822(72)80002-3" @default.
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