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- W2093687946 abstract "In the previous paper (Hell, 1964), we described the in vitro culture of the de-embryonated chick blastoderm. This system was shown to be suitable for the observation of the differentiation of the mesodermal blood islands, and of initial haemoglobin (Hb) synthesis. O9Brien (1961) had shown that Hb formation in such blastodiscs could be inhibited if azaguanine was added to the culture medium before and up to a time of development equivalent to the 4 somite stage (stage 8- on the Hamburger & Hamilton scale, 1951), while if this RNA precursor analogue was added to cultures of explants de-embryonated at 7 somites or later, it had barely any effect. Similarly, he showed that fluorophenylalanine caused a considerable inhibition of Hb synthesis if applied up to 7 somites. We have followed up this work, both with morphological studies and with biochemical analyses, to determine to what extent the effects were specific to Hb synthesis." @default.
- W2093687946 created "2016-06-24" @default.
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- W2093687946 date "1964-12-01" @default.
- W2093687946 modified "2023-09-23" @default.
- W2093687946 title "THE INITIAL SYNTHESIS OF HAEMOGLOBIN IN DE-EMBRYONATED CHICK BLASTODERMS. II. THE EFFECT OF METABOLIC INHIBITORS ON THE BLASTODISC CULTURED IN VITRO." @default.
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