Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093701618> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2093701618 endingPage "112" @default.
- W2093701618 startingPage "105" @default.
- W2093701618 abstract "SUMMARY. A flow-cytometric method was developed to determine the number of molecules of IgG bound to D-positive red blood cells (RBC) when sensitized with low plasma concentrations of IgG anti-D in the presence of an excess of D-negative RBC. D-positive RBC were infused into 12 D-negative male volunteers 2 days after intramuscular injection of monoclonal anti-D (BRAD-3, IgG3 or BRAD-5, IgG1). Blood samples were taken immediately before, 3 min and 3 h after injection of the RBC, incubated for 1 h at 37°C, washed, then incubated sequentially with FITC-conjugated anti-IgG, IgM monoclonal anti-D, biotin-conjugated anti-IgM, and R-phycoerythrin-conjugated streptavidin. To prepare a standard curve, O R1R2 RBC were incubated in duplicate with varying dilutions of BRAD-3 or BRAD-5, and RBC from one set were mixed with an excess of D-negative RBC (1:100) and labelled as above, while cell-bound IgG on the other set was quantified by ELISA. The test samples and standards were analysed by flow cytometry and the mean channel (FITC) fluorescence was plotted against molecules IgG/cell, from which the sensitization level of D-positive RBC in the test samples was determined. The use of IgM anti-D enhanced the discrimination between D-positive and D-negative RBC, especially when fewer than about 3000 molecules IgG/cell were bound. The assay was sensitive to about 1000 molecules IgG/cell. The sensitization levels of the D-positive RBC in samples taken 3 h after injection were found to be in the range 1500-10,000 molecules IgG anti-D per cell." @default.
- W2093701618 created "2016-06-24" @default.
- W2093701618 creator A5006013394 @default.
- W2093701618 creator A5039353224 @default.
- W2093701618 date "1995-06-01" @default.
- W2093701618 modified "2023-09-27" @default.
- W2093701618 title "Quantification of IgG anti-D bound to D-positive red cells infused into D-negative subjects after intramuscular injection of monoclonal anti-D" @default.
- W2093701618 cites W13822348 @default.
- W2093701618 cites W1965863276 @default.
- W2093701618 cites W1971294201 @default.
- W2093701618 cites W1987248924 @default.
- W2093701618 cites W2006995345 @default.
- W2093701618 cites W2011133091 @default.
- W2093701618 cites W2042008525 @default.
- W2093701618 cites W2046823101 @default.
- W2093701618 cites W2058448367 @default.
- W2093701618 cites W2062965580 @default.
- W2093701618 cites W2068327647 @default.
- W2093701618 cites W2069006179 @default.
- W2093701618 cites W2081185550 @default.
- W2093701618 cites W2081785583 @default.
- W2093701618 cites W2091786682 @default.
- W2093701618 cites W2116131342 @default.
- W2093701618 cites W2411340820 @default.
- W2093701618 doi "https://doi.org/10.1111/j.1365-3148.1995.tb00196.x" @default.
- W2093701618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7655572" @default.
- W2093701618 hasPublicationYear "1995" @default.
- W2093701618 type Work @default.
- W2093701618 sameAs 2093701618 @default.
- W2093701618 citedByCount "20" @default.
- W2093701618 countsByYear W20937016182013 @default.
- W2093701618 crossrefType "journal-article" @default.
- W2093701618 hasAuthorship W2093701618A5006013394 @default.
- W2093701618 hasAuthorship W2093701618A5039353224 @default.
- W2093701618 hasConcept C153911025 @default.
- W2093701618 hasConcept C159654299 @default.
- W2093701618 hasConcept C163864487 @default.
- W2093701618 hasConcept C185592680 @default.
- W2093701618 hasConcept C203014093 @default.
- W2093701618 hasConcept C2776190903 @default.
- W2093701618 hasConcept C2776217527 @default.
- W2093701618 hasConcept C2776557347 @default.
- W2093701618 hasConcept C2778204606 @default.
- W2093701618 hasConcept C542903549 @default.
- W2093701618 hasConcept C553184892 @default.
- W2093701618 hasConcept C55493867 @default.
- W2093701618 hasConcept C71924100 @default.
- W2093701618 hasConcept C86803240 @default.
- W2093701618 hasConceptScore W2093701618C153911025 @default.
- W2093701618 hasConceptScore W2093701618C159654299 @default.
- W2093701618 hasConceptScore W2093701618C163864487 @default.
- W2093701618 hasConceptScore W2093701618C185592680 @default.
- W2093701618 hasConceptScore W2093701618C203014093 @default.
- W2093701618 hasConceptScore W2093701618C2776190903 @default.
- W2093701618 hasConceptScore W2093701618C2776217527 @default.
- W2093701618 hasConceptScore W2093701618C2776557347 @default.
- W2093701618 hasConceptScore W2093701618C2778204606 @default.
- W2093701618 hasConceptScore W2093701618C542903549 @default.
- W2093701618 hasConceptScore W2093701618C553184892 @default.
- W2093701618 hasConceptScore W2093701618C55493867 @default.
- W2093701618 hasConceptScore W2093701618C71924100 @default.
- W2093701618 hasConceptScore W2093701618C86803240 @default.
- W2093701618 hasIssue "2" @default.
- W2093701618 hasLocation W20937016181 @default.
- W2093701618 hasLocation W20937016182 @default.
- W2093701618 hasOpenAccess W2093701618 @default.
- W2093701618 hasPrimaryLocation W20937016181 @default.
- W2093701618 hasRelatedWork W2067077431 @default.
- W2093701618 hasRelatedWork W2087041936 @default.
- W2093701618 hasRelatedWork W2091594406 @default.
- W2093701618 hasRelatedWork W2368460920 @default.
- W2093701618 hasRelatedWork W2380360044 @default.
- W2093701618 hasRelatedWork W2382797166 @default.
- W2093701618 hasRelatedWork W2410111069 @default.
- W2093701618 hasRelatedWork W2417465673 @default.
- W2093701618 hasRelatedWork W4252441337 @default.
- W2093701618 hasRelatedWork W2101238374 @default.
- W2093701618 hasVolume "5" @default.
- W2093701618 isParatext "false" @default.
- W2093701618 isRetracted "false" @default.
- W2093701618 magId "2093701618" @default.
- W2093701618 workType "article" @default.