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- W2093741783 abstract "A membrane-bound phosphatidylinositol 4-kinase (PtdIns kinase) has been purified to apparent homogeneity from human erythrocytes. Enzyme activity was solubilized from urea-KCl-stripped, inside-out membrane vesicles by 3% Triton X-100. Purification to apparent homogeneity was accomplished by cation-exchange chromatography on phosphocellulose, followed by heparin-acrylamide chromatography. This resulted in a nearly 3900-fold purification of PtdIns kinase activity to a specific activity of 44 nmol min −1 mg −1 . The purified enzyme has an M r of 59 000 on silver-stained SDS-PAGE; however, many preparations also contain 54 kDa and 50 kDa proteins which are related to the 59 kDa protein and have PtdIns kinase activity. Kinetic analysis of the PtdIns kinase indicate apparent K m values of 40 and 35 μM for phosphatidylinositol and ATP, respectively. The purified enzyme has been reconstituted into phospholipid liposomes and shown to phosphorylate phosphatidylinositol." @default.
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- W2093741783 date "1991-10-01" @default.
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- W2093741783 title "Purification and reconstitution of phosphatidylinositol 4-kinase from human erythrocytes" @default.
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- W2093741783 doi "https://doi.org/10.1016/0167-4838(91)90105-9" @default.
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