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• W2093742154 endingPage "2129" @default.
• W2093742154 startingPage "2120" @default.
• W2093742154 abstract "The robustness of the popular ‘Tanaka’ LC column characterization protocol has been evaluated by the statistical tools of reduced factorial design, multiple linear regression and principal component analysis. These have shown that in order to obtain reliable and reproducible results, it is especially important to control the methanol content, the temperature and, in the case of the total ion-exchange capacity test (αB/P pH 7.6), the pH of the mobile phase. In particular, the hydrophobicity tests (kPB and αequation/tex2gif-inf-3.gif) are sensitive to small changes in methanol content. Provided that the operating parameters for the Tanaka column characterization protocol are controlled within the following experimental limits, i. e. methanol content ±0.5% v/v, temperature ±3°C, pH ±0.10 and buffer concentration ±2.0 mM, it is feasible to distinguish between RP materials that possess selectivity differences larger than their batch-to-batch reproducibility. These experimental requirements can be easily met by current LC instrumentation. Hence, the validity of the Tanaka testing protocol for characterizing columns has been verified." @default.
• W2093742154 created "2016-06-24" @default.
• W2093742154 creator A5049131182 @default.
• W2093742154 creator A5075362296 @default.
• W2093742154 date "2005-10-01" @default.
• W2093742154 modified "2023-09-23" @default.
• W2093742154 title "An evaluation of the robustness of the Tanaka characterization protocol for reversed-phase liquid chromatography columns" @default.
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• W2093742154 doi "https://doi.org/10.1002/jssc.200500151" @default.
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