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- W2093766534 abstract "When cells are cryopreserved in programmed cooling machines, they supercool to a variable and uncontrolled extent. Experiments were carried out with three cell-types (human peripheral lymphocytes, Chinese hamster lung fibroblasts, and mouse lymphoma cells) to determine whether there was any effect of supercooling on cell survival. Samples were cooled at 1 °C min−1 in the presence of 12% v/v dimethyl sulphoxide (Me2SO) to −100 °C, and then thawed rapidly in a 37 °C water bath. There was no correlation between the extent of supercooling or the maximum cooling rate after freezing and cell survival, but the time taken for the sample temperature to return to the temperature at which freezing occurred did influence the survival of the two tissue culture cell lines. These results are interpreted on the basis of current theories according to which cells require sufficient time to lose water as they cool in order to avoid subsquent intracellular freezing, but must be cooled sufficiently rapidly to minimise solution effects. It is concluded that the variations in supercooling that occur in programmed cooling machines present no particular difficulties, providing appropriate cooling rates are chosen." @default.
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- W2093766534 date "1979-08-01" @default.
- W2093766534 modified "2023-09-23" @default.
- W2093766534 title "Cell preservation in a programmed cooling machine: The effect of variations in supercooling" @default.
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- W2093766534 doi "https://doi.org/10.1016/0011-2240(79)90043-9" @default.
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