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• W2093777660 endingPage "47" @default.
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• W2093777660 abstract "DNA polymerases α, δ and ϵ were partially purified and characterized from a wild type Chinese hamster ovary (CHO) cell line and from two aphidicolin-resistant mutant CHO cell lines (BR5 and BR5-20a). The main characteristics of the wild type and mutant DNA polymerases were compared in order to reveal differences in the properties of these enzymes responsible for the aphidicolin resistance of the mutant cell lines. Pol α's of the mutant cells show: (1) in vitro aphidicolin-resistance, (2) 1.5–3-fold lower specific activity than that of the wild type, (3) resistance to cytosine and adenosine arabinofuranoside 5′-triphosphate (araCTP and araATP), (4) altered resistance to carbonyldiphosphonate (COMDP) and to alkylphenyl nucleotide analogs (butylphenyl-dGTP and butylanilino-dATP), and (5) lower activity on poly(dA)/oligo(dT) template-primers. These changes in the biochemical properties of this enzyme may result from a mutation in pol α gene. Pol ϵ and δ of the mutant cells did not differ from the wild type enzymes with respect to aphidicolin resistance. However, the specific activities of these mutant enzymes were much higher (1.5 to 8-fold for pol ϵ and 4 to 20-fold for pol δ) in comparison to that of the wild type enzymes. Also in comparison to the wild type enzymes, the mutant pol ϵ showed changes in the template-primer preference; whereas the mutant pol δ was found to have altered sensitivity to other inhibitors. These results indicate that pol ϵ and pol δ are also altered as a secondary effect of mutation in the aphidicolin-resistant cells. It is suggested that these altered properties of the DNA pols of the α family are responsible for the in vivo aphidicolin resistance of the mutant cells." @default.
• W2093777660 created "2016-06-24" @default.
• W2093777660 creator A5029137576 @default.
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• W2093777660 date "1994-05-01" @default.
• W2093777660 modified "2023-09-27" @default.
• W2093777660 title "Aphidicolin-resistant Chinese hamster ovary cells possess altered DNA polymerases of the α-family" @default.
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• W2093777660 doi "https://doi.org/10.1016/0167-4781(94)90098-1" @default.
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