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- W2093847450 abstract "In order to optimize the detection and measurement of α-melanocyte-stimulating hormone (α-MSH) receptivity in human melanoma cells, and the authors replaced the natural hormone by [Nle4,D-Phe7]-α-MSH, a more stable and potent analogue in the receptor binding assay commonly performed with α-MSH. The following parameters were investigated: temperature, incubation time, number of cells, and ratio of labelled to unlabelled hormone. Optimal conditions for each assay were determined. The results demonstrate that the analogue has identical binding sites to α-MSH, as similar reciprocal displacements of each labelled (125I) hormone by serial dilutions of unlabelled α-MSH or [Nle4,D-Phe7]-α-MSH (10−12 M to 10−6 M) were obtained. To further compare the two hormones, we performed a screening of various human cell lines: ten melanomas and five nonmelanomas. The assay with [Nle4,D-Phe7]-α-MSH yielded more receptor expression on six often melanoma lines against only four often with the natural hormone. In conclusion, the use of radiolabelled [Nle4,D-Phe7]-α-MSH analogue instead of labelled α-MSH improved both sensitivity and reproducibility in this receptor binding assay on human melanoma lines." @default.
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- W2093847450 date "1989-11-01" @default.
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- W2093847450 title "Use of an Alpha-Melanocyte-Stimulating Hormone Analogue to Improve Alpha-Melanocyte-Stimulating Hormone Receptor Binding Assay in Human Melanoma" @default.
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- W2093847450 doi "https://doi.org/10.1111/j.1600-0749.1989.tb00247.x" @default.
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