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- W2093875269 abstract "Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac146 is a highly conserved gene in the Alpha- and Betabaculovirus genera that has an unknown function. Northern blot analysis and transcript mapping showed that ac146 is transcribed at late times post infection as a 1.2 kb mRNA. To determine the role of ac146 in the baculovirus life cycle ac146 knock out viruses were constructed. Transfection and plaque assays showed that all the ac146 deletions produced a single cell phenotype indicating that no infectious budded virus (BV) was produced, however occlusion bodies were formed. The lack of BV production was confirmed by viral titration utilizing both qPCR and TCID50. Analysis of BV and occlusion derived virus (ODV) revealed that AC146 is associated with both forms of the virus and is modified specifically in ODV. This study therefore demonstrates that AC146 is a late virion associated protein and is essential for the viral life cycle." @default.
- W2093875269 created "2016-06-24" @default.
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- W2093875269 date "2012-09-01" @default.
- W2093875269 modified "2023-10-03" @default.
- W2093875269 title "Deletion of AcMNPV ac146 eliminates the production of budded virus" @default.
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- W2093875269 doi "https://doi.org/10.1016/j.virol.2012.05.002" @default.
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