FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093907378> ?p ?o ?g. }

• W2093907378 endingPage "1003" @default.
• W2093907378 startingPage "998" @default.
• W2093907378 abstract "In the present study, a culture system of human placental cells was established to examine the role of estrogen and androgen in progesterone (P4) formation. Normal human placentae were obtained at term, and cells were dispersed in Hank′s Balanced Salt Solution (5 ml/g tissue) containing 0.1% collagenase, 0.1% hyaluronidase, 0.01% deoxyribonuclease, and 1% fetal bovine serum for 2 h at 37 C. Dispersed placental cells (108 cells/ml) were placed in medium 199 with modified Earle′s salts (pH 7.4) containing 10% fetal bovine serum, 12.5 mm HEPES buffer, 26 mm NaHCO3, and 40 μg/ml Gentamycin-SO4 and incubated for 72 h at 37 C and 5% CO2 in air to allow cell attachment. Medium was then changed (time zero), and P4 formation was studied thereafter. Culture of placental cells for 96 h resulted in linear increases in P4 and estradiol (E2) formation, indicating the maintenance of cell viability and steroidogenic function. Mean ± SE P4 formation at 48 h was 246 ± 16 pg/μg DNA. To assess the role of estrogen on P4 formation, placental cells were incubated for a period of 48 h with various amounts (10-7-10-4m) of the antiestrogen ethamoxytriphetol (MER-25), the aromatase inhibitor 4- hydroxyandrostenedione (4-OHA), and/or E2. Both MER-25 and 4-OHA resulted in a dose-dependent decline (P < 0.01) in P4 formation (80% decline at 10-4m MER-25 or 4-OHA). The marked reduction in P4 formation caused by 4-OHA alone was reversed by concomitant addition of E2 however, E2 alone had no effect. To assess the role of androgens on P4 formation, cells were incubated for 48 h with increasing amounts (10-7-10-4m) of androstenedione, dehydroepiandrosterone (DHA), or dihydrotestosterone. Although the formation of E2 was enhanced by DHA, formation of P4 was not affected by the aromatizable androgens DHA or androstenedione or the nonaromatizable dihydrotestosterone. The decline in P4 formation by human placental cells in culture elicited by MER-25 or 4-OHA supports the hypothesis of a regulatory role for estrogen in placental P4 formation during human pregnancy. The lack of effect of exogenous estrogen suggests that the action of estrogen on P4 formation may be permissive. (Endocrinology119: 998-1003, 1986)" @default.
• W2093907378 created "2016-06-24" @default.
• W2093907378 creator A5057078720 @default.
• W2093907378 creator A5060010329 @default.
• W2093907378 creator A5067404225 @default.
• W2093907378 creator A5082314899 @default.
• W2093907378 date "1986-09-01" @default.
• W2093907378 modified "2023-10-11" @default.
• W2093907378 title "Regulation of Progesterone Formation by Human Placental Cells in Culture*" @default.
• W2093907378 cites W1505555004 @default.
• W2093907378 cites W1546029719 @default.
• W2093907378 cites W1761890410 @default.
• W2093907378 cites W190777749 @default.
• W2093907378 cites W1965577999 @default.
• W2093907378 cites W1981545857 @default.
• W2093907378 cites W1998183746 @default.
• W2093907378 cites W2067680882 @default.
• W2093907378 cites W2072633671 @default.
• W2093907378 cites W2077323442 @default.
• W2093907378 cites W2080063136 @default.
• W2093907378 cites W2086383375 @default.
• W2093907378 cites W2115991431 @default.
• W2093907378 cites W2121209426 @default.
• W2093907378 cites W2144655531 @default.
• W2093907378 cites W2410335316 @default.
• W2093907378 cites W2412143391 @default.
• W2093907378 doi "https://doi.org/10.1210/endo-119-3-998" @default.
• W2093907378 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2942394" @default.
• W2093907378 hasPublicationYear "1986" @default.
• W2093907378 type Work @default.
• W2093907378 sameAs 2093907378 @default.
• W2093907378 citedByCount "30" @default.
• W2093907378 countsByYear W20939073782015 @default.
• W2093907378 countsByYear W20939073782022 @default.
• W2093907378 crossrefType "journal-article" @default.
• W2093907378 hasAuthorship W2093907378A5057078720 @default.
• W2093907378 hasAuthorship W2093907378A5060010329 @default.
• W2093907378 hasAuthorship W2093907378A5067404225 @default.
• W2093907378 hasAuthorship W2093907378A5082314899 @default.
• W2093907378 hasConcept C126322002 @default.
• W2093907378 hasConcept C134018914 @default.
• W2093907378 hasConcept C16685009 @default.
• W2093907378 hasConcept C172680121 @default.
• W2093907378 hasConcept C181199279 @default.
• W2093907378 hasConcept C185592680 @default.
• W2093907378 hasConcept C2777164284 @default.
• W2093907378 hasConcept C2778394429 @default.
• W2093907378 hasConcept C2779234561 @default.
• W2093907378 hasConcept C2779404806 @default.
• W2093907378 hasConcept C2780375986 @default.
• W2093907378 hasConcept C54355233 @default.
• W2093907378 hasConcept C55493867 @default.
• W2093907378 hasConcept C71924100 @default.
• W2093907378 hasConcept C81885089 @default.
• W2093907378 hasConcept C86803240 @default.
• W2093907378 hasConceptScore W2093907378C126322002 @default.
• W2093907378 hasConceptScore W2093907378C134018914 @default.
• W2093907378 hasConceptScore W2093907378C16685009 @default.
• W2093907378 hasConceptScore W2093907378C172680121 @default.
• W2093907378 hasConceptScore W2093907378C181199279 @default.
• W2093907378 hasConceptScore W2093907378C185592680 @default.
• W2093907378 hasConceptScore W2093907378C2777164284 @default.
• W2093907378 hasConceptScore W2093907378C2778394429 @default.
• W2093907378 hasConceptScore W2093907378C2779234561 @default.
• W2093907378 hasConceptScore W2093907378C2779404806 @default.
• W2093907378 hasConceptScore W2093907378C2780375986 @default.
• W2093907378 hasConceptScore W2093907378C54355233 @default.
• W2093907378 hasConceptScore W2093907378C55493867 @default.
• W2093907378 hasConceptScore W2093907378C71924100 @default.
• W2093907378 hasConceptScore W2093907378C81885089 @default.
• W2093907378 hasConceptScore W2093907378C86803240 @default.
• W2093907378 hasIssue "3" @default.
• W2093907378 hasLocation W20939073781 @default.
• W2093907378 hasLocation W20939073782 @default.
• W2093907378 hasOpenAccess W2093907378 @default.
• W2093907378 hasPrimaryLocation W20939073781 @default.
• W2093907378 hasRelatedWork W1964476659 @default.
• W2093907378 hasRelatedWork W1993100526 @default.
• W2093907378 hasRelatedWork W2011542082 @default.
• W2093907378 hasRelatedWork W2103258952 @default.
• W2093907378 hasRelatedWork W2110910266 @default.
• W2093907378 hasRelatedWork W2156492564 @default.
• W2093907378 hasRelatedWork W2188510251 @default.
• W2093907378 hasRelatedWork W2394141215 @default.
• W2093907378 hasRelatedWork W2410261867 @default.
• W2093907378 hasRelatedWork W2417833920 @default.
• W2093907378 hasVolume "119" @default.
• W2093907378 isParatext "false" @default.
• W2093907378 isRetracted "false" @default.
• W2093907378 magId "2093907378" @default.
• W2093907378 workType "article" @default.