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- W2093908082 abstract "<p>The aim of this study was to determine the influence of <em>meta</em>-topolin (<em>m</em>T) and 6-benzyl-aminopurine (BAP) on the hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) level and antioxidant enzymes activities in relation to the shoot formation and senescence process in <em>Pelargonium hortorum</em> cultivars, which differ in their susceptibility to leaf yellowing under <em>in vitro</em> conditions.</p><p>In an early senescing cultivar ‘Grand Prix’, the addition of an aromatic cytokinin <em>m</em>T to abscisic acid (ABA)-enriched Murashige and Skoog (MS) basal medium more efficiently inhibited leaf yellowing than BAP. In both genotypes, <em>meta</em>-topolin was also the most effective in shoot formation. It was found that <em>Pelargonium</em> species varying in their susceptibility to senescence differ in H<sub>2</sub>O<sub>2</sub> production and antioxidant enzymes activities. Generally, <em>meta</em>-topolin more effectively enhanced H<sub>2</sub>O<sub>2 </sub>production and POD activity than BAP and control medium, but its effect depended on genotype. The highest H<sub>2</sub>O<sub>2</sub> production stimulated by <em>m</em>T was observed on day 5 of subculture in late senescing cv. ‘Bergpalais’. In both geranium genotypes, superoxide dismutase (SOD) and catalase (CAT) levels were highest at the beginning of the subculture period, during the initiation of shoot formation. SOD showed the highest activity on day 5 of subculture on the medium without cytokinin and generally being higher in cv. ‘Bergpalais’ than in cv. ‘Grand Prix’. CAT activity was positively regulated by both cytokinins. POD activity was most effectively enhanced by <em>m</em>T, but on different days of subculture - on the 2<sup>nd</sup> day of subculture in cv. ‘Bergpalais’ and on the 22<sup>nd</sup> day of subculture in cv. ‘Grand Prix’. The enhanced activity of POD in the presence of <em>m</em>T, 4-fold higher than on control medium, at the end of subculture in <em>P. hortorum </em>‘Grand Prix’ coincided with the inhibition of leaf senescence.</p>" @default.
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- W2093908082 date "2014-01-01" @default.
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- W2093908082 title "Effects of cytokinins on antioxidant enzymes in in vitro grown shoots of Pelargonium hortorum L. H. Bayley" @default.
- W2093908082 doi "https://doi.org/10.5586/aa.2014.042" @default.
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