FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093949819> ?p ?o ?g. }

• W2093949819 abstract "Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease of the axial skeleton, characterised by systemic osteoporosis along with new local bone formation. Previous studies have shown that serum levels of TNF, IL-6 and IL-17 are increased in AS patients and may be implicated in the development of secondary osteoporosis, since these cytokines are able to induce osteoclast (OC) differentiation and, therefore, bone resorption. In this work we aimed to assess the effects of TNF-blocking therapy in the systemic inflammatory environment of AS patients with active disease as well as in OC differentiation and activity. Patients with AS starting TNF-blocking therapy were recruited for this study and blood was collected at baseline and 6 months after the first treatment administration. We performed ELISAs in the serum of these patients to assess cytokine levels and bone turnover markers. We also characterised RANKL surface expression in circulating neutrophils, B and T lymphocytes and monocyte subpopulation frequency and phenotype by flow cytometry. We cultured circulating monocytes from AS patients, before and after therapy, and from healthy controls under osteoclastogenic conditions. We performed two functional assays (TRAP staining and resorption pit assay) and analysed the expression of osteoclast specific genes. We found no differences (before and after treatment) in any of the circulating monocytes’ subpopulations regarding frequency or cell death assessed by annexin staining. Phenotype analysis only revealed differences in the classical monocyte subpopulation where there was a significant decrease in HLA-DR surface expression after treatment. The frequency of RANKL positive B lymphocytes was reduced after treatment. No changes were observed on RANKL expression in neutrophils or T lymphocytes after treatment. Before TNF-blocking treatment AS patients have increased levels of pro-inflammatory cytokines when compared with healthy subjects. After TNF-blocking therapy IL-17, TGF-β and osteoprotegerin were significantly decreased. Interestingly, we observed that after TNF-blocking therapy, gene expression was favouring osteoclastogenesis and that differentiated OCs have increased resorption activity. Our results suggest that in AS patients there might be a paradoxical effect of TNF blocking therapy that induces OC activity." @default.
• W2093949819 created "2016-06-24" @default.
• W2093949819 creator A5002804464 @default.
• W2093949819 creator A5002875386 @default.
• W2093949819 creator A5009420603 @default.
• W2093949819 creator A5010764188 @default.
• W2093949819 creator A5019866571 @default.
• W2093949819 creator A5048823949 @default.
• W2093949819 creator A5050646807 @default.
• W2093949819 creator A5069235693 @default.
• W2093949819 date "2015-06-01" @default.
• W2093949819 modified "2023-10-05" @default.
• W2093949819 title "FRI0192 Effect of TNF Blocking Therapy on Osteoclasts from Ankylosing Spondylitis Patients" @default.
• W2093949819 doi "https://doi.org/10.1136/annrheumdis-2015-eular.3088" @default.
• W2093949819 hasPublicationYear "2015" @default.
• W2093949819 type Work @default.
• W2093949819 sameAs 2093949819 @default.
• W2093949819 citedByCount "0" @default.
• W2093949819 crossrefType "journal-article" @default.
• W2093949819 hasAuthorship W2093949819A5002804464 @default.
• W2093949819 hasAuthorship W2093949819A5002875386 @default.
• W2093949819 hasAuthorship W2093949819A5009420603 @default.
• W2093949819 hasAuthorship W2093949819A5010764188 @default.
• W2093949819 hasAuthorship W2093949819A5019866571 @default.
• W2093949819 hasAuthorship W2093949819A5048823949 @default.
• W2093949819 hasAuthorship W2093949819A5050646807 @default.
• W2093949819 hasAuthorship W2093949819A5069235693 @default.
• W2093949819 hasConcept C105795698 @default.
• W2093949819 hasConcept C126322002 @default.
• W2093949819 hasConcept C144745244 @default.
• W2093949819 hasConcept C17991360 @default.
• W2093949819 hasConcept C2777226972 @default.
• W2093949819 hasConcept C2777402515 @default.
• W2093949819 hasConcept C2779650986 @default.
• W2093949819 hasConcept C2779722408 @default.
• W2093949819 hasConcept C33923547 @default.
• W2093949819 hasConcept C71924100 @default.
• W2093949819 hasConceptScore W2093949819C105795698 @default.
• W2093949819 hasConceptScore W2093949819C126322002 @default.
• W2093949819 hasConceptScore W2093949819C144745244 @default.
• W2093949819 hasConceptScore W2093949819C17991360 @default.
• W2093949819 hasConceptScore W2093949819C2777226972 @default.
• W2093949819 hasConceptScore W2093949819C2777402515 @default.
• W2093949819 hasConceptScore W2093949819C2779650986 @default.
• W2093949819 hasConceptScore W2093949819C2779722408 @default.
• W2093949819 hasConceptScore W2093949819C33923547 @default.
• W2093949819 hasConceptScore W2093949819C71924100 @default.
• W2093949819 hasLocation W20939498191 @default.
• W2093949819 hasOpenAccess W2093949819 @default.
• W2093949819 hasPrimaryLocation W20939498191 @default.
• W2093949819 hasRelatedWork W1516746560 @default.
• W2093949819 hasRelatedWork W1980199917 @default.
• W2093949819 hasRelatedWork W2018410379 @default.
• W2093949819 hasRelatedWork W2027482179 @default.
• W2093949819 hasRelatedWork W2067316183 @default.
• W2093949819 hasRelatedWork W2130336393 @default.
• W2093949819 hasRelatedWork W2151028032 @default.
• W2093949819 hasRelatedWork W2398756499 @default.
• W2093949819 hasRelatedWork W2977230696 @default.
• W2093949819 hasRelatedWork W4235629817 @default.
• W2093949819 isParatext "false" @default.
• W2093949819 isRetracted "false" @default.
• W2093949819 magId "2093949819" @default.
• W2093949819 workType "article" @default.