Matches in SemOpenAlex for { <https://semopenalex.org/work/W2093973257> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2093973257 endingPage "63" @default.
- W2093973257 startingPage "57" @default.
- W2093973257 abstract "The current study aims to investigate the pharmacokinetics and brain disposition of tacrine and its three major bioactive monohydroxylated metabolites (1-hydroxytacrine, 2-hydroxytacrine and 4-hydroxytacrine). An assay for simultaneous quantification of tacrine and three above metabolites in rat plasma and brain tissue was developed. Four analytes together with internal standard were extracted from rat plasma or brain tissue homogenate by liquid–liquid extraction using ethyl acetate. Baseline separation of the four studied compounds was achieved by a Thermo Hypersil BDS C18 column with gradient elution using acetonitrile and ammonium formate-triethylamine (pH 4.0) under fluorescence detection. Extraction recoveries of all analytes ranged from 82.1% to 93.2% in both rat plasma and brain tissue. The intra- and inter-day precision and accuracy of each analyte at lower limit of quantification (LLOQ) and three quality control (QC) concentrations (low, middle and high) was within 12% RSD and within 11% bias in both biological matrices. The LLOQ for tacrine, 1-hydroxytacrine, 2-hydroxytacrine and 4-hydroxytacrine were found to be 2.5, 6.7, 2.1 and 2.1 ng/ml in plasma, and 12.3, 33.5, 10.6 and 10.5 ng/g in brain tissue, respectively. All four analytes were stable during analysis. The developed method provides a simple, sensitive and reproducible procedure for pharmacokinetics and brain disposition study of tacrine and its three major metabolites in rats after oral administration of tacrine. Pharmacokinetic study demonstrated that tacrine could be quickly absorbed and extensively metabolized to its monohydroxylated metabolites in vivo. In addition, rat brain disposition study showed that tacrine and its monohydroxylated metabolites were evenly distributed in different brain regions except for a slight lower concentration of tacrine in olfactory region. Moreover, tacrine and 4-hydroxytacrine exhibited a much higher brain-to-plasma ratio than that of 1-hydroxytacrine and 2-hydroxytacrine." @default.
- W2093973257 created "2016-06-24" @default.
- W2093973257 creator A5033792438 @default.
- W2093973257 creator A5077204577 @default.
- W2093973257 creator A5081004832 @default.
- W2093973257 date "2012-03-01" @default.
- W2093973257 modified "2023-10-16" @default.
- W2093973257 title "Pharmacokinetics and brain dispositions of tacrine and its major bioactive monohydroxylated metabolites in rats" @default.
- W2093973257 cites W1503903593 @default.
- W2093973257 cites W1706691858 @default.
- W2093973257 cites W1967568501 @default.
- W2093973257 cites W1969000896 @default.
- W2093973257 cites W1981320103 @default.
- W2093973257 cites W1982556979 @default.
- W2093973257 cites W1991865398 @default.
- W2093973257 cites W1992829796 @default.
- W2093973257 cites W2034055025 @default.
- W2093973257 cites W2053694461 @default.
- W2093973257 cites W2053972194 @default.
- W2093973257 cites W2059937283 @default.
- W2093973257 cites W2060609593 @default.
- W2093973257 cites W2062965366 @default.
- W2093973257 cites W2071161708 @default.
- W2093973257 cites W207133542 @default.
- W2093973257 cites W2078592950 @default.
- W2093973257 cites W2096821680 @default.
- W2093973257 cites W2112882070 @default.
- W2093973257 cites W2142541344 @default.
- W2093973257 cites W2169812253 @default.
- W2093973257 cites W2335206571 @default.
- W2093973257 cites W2468464101 @default.
- W2093973257 doi "https://doi.org/10.1016/j.jpba.2011.11.025" @default.
- W2093973257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22196801" @default.
- W2093973257 hasPublicationYear "2012" @default.
- W2093973257 type Work @default.
- W2093973257 sameAs 2093973257 @default.
- W2093973257 citedByCount "18" @default.
- W2093973257 countsByYear W20939732572012 @default.
- W2093973257 countsByYear W20939732572013 @default.
- W2093973257 countsByYear W20939732572014 @default.
- W2093973257 countsByYear W20939732572016 @default.
- W2093973257 countsByYear W20939732572017 @default.
- W2093973257 countsByYear W20939732572019 @default.
- W2093973257 countsByYear W20939732572020 @default.
- W2093973257 countsByYear W20939732572021 @default.
- W2093973257 countsByYear W20939732572022 @default.
- W2093973257 countsByYear W20939732572023 @default.
- W2093973257 crossrefType "journal-article" @default.
- W2093973257 hasAuthorship W2093973257A5033792438 @default.
- W2093973257 hasAuthorship W2093973257A5077204577 @default.
- W2093973257 hasAuthorship W2093973257A5081004832 @default.
- W2093973257 hasConcept C10390740 @default.
- W2093973257 hasConcept C112705442 @default.
- W2093973257 hasConcept C179998833 @default.
- W2093973257 hasConcept C181199279 @default.
- W2093973257 hasConcept C185592680 @default.
- W2093973257 hasConcept C2775986502 @default.
- W2093973257 hasConcept C2778816929 @default.
- W2093973257 hasConcept C2779046751 @default.
- W2093973257 hasConcept C43617362 @default.
- W2093973257 hasConcept C4725764 @default.
- W2093973257 hasConcept C55493867 @default.
- W2093973257 hasConcept C71924100 @default.
- W2093973257 hasConcept C98274493 @default.
- W2093973257 hasConceptScore W2093973257C10390740 @default.
- W2093973257 hasConceptScore W2093973257C112705442 @default.
- W2093973257 hasConceptScore W2093973257C179998833 @default.
- W2093973257 hasConceptScore W2093973257C181199279 @default.
- W2093973257 hasConceptScore W2093973257C185592680 @default.
- W2093973257 hasConceptScore W2093973257C2775986502 @default.
- W2093973257 hasConceptScore W2093973257C2778816929 @default.
- W2093973257 hasConceptScore W2093973257C2779046751 @default.
- W2093973257 hasConceptScore W2093973257C43617362 @default.
- W2093973257 hasConceptScore W2093973257C4725764 @default.
- W2093973257 hasConceptScore W2093973257C55493867 @default.
- W2093973257 hasConceptScore W2093973257C71924100 @default.
- W2093973257 hasConceptScore W2093973257C98274493 @default.
- W2093973257 hasLocation W20939732571 @default.
- W2093973257 hasLocation W20939732572 @default.
- W2093973257 hasOpenAccess W2093973257 @default.
- W2093973257 hasPrimaryLocation W20939732571 @default.
- W2093973257 hasRelatedWork W1588095382 @default.
- W2093973257 hasRelatedWork W1973892552 @default.
- W2093973257 hasRelatedWork W2008160846 @default.
- W2093973257 hasRelatedWork W2018671480 @default.
- W2093973257 hasRelatedWork W2045265325 @default.
- W2093973257 hasRelatedWork W2077423873 @default.
- W2093973257 hasRelatedWork W2093917715 @default.
- W2093973257 hasRelatedWork W2376531500 @default.
- W2093973257 hasRelatedWork W2601524031 @default.
- W2093973257 hasRelatedWork W3200692342 @default.
- W2093973257 hasVolume "61" @default.
- W2093973257 isParatext "false" @default.
- W2093973257 isRetracted "false" @default.
- W2093973257 magId "2093973257" @default.
- W2093973257 workType "article" @default.