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- W2094037158 abstract "The adaptor complexes AP-1 and AP-3 are localized to endosomes and/or the trans Golgi network (TGN). Because of limitations in analysing intracellular adaptor function directly, their site of function is a matter of ongoing uncertainty. To overcome this problem and to analyse adaptor sorting at the TGN, we reconstituted vesicle formation from Golgi/TGN-enriched membranes in a novel in vitro budding assay. Melanocytes were metabolically labelled followed by a 19 degrees C temperature block to accumulate newly synthesized proteins in Golgi membranes, which were then enriched by subcellular fractionation and used as donor membranes for vesicle formation in vitro. The incorporation of the melanosomal proteins tyrosinase and tyrosinase-related protein 1 (TRP-1) as well as Lamp-1 and 46 kDa mannose-6-phosphate receptor (MPR46) into Golgi/TGN-derived vesicles was temperature, nucleotide, cytosol, ADP ribosylation factor 1 and adaptor dependent. We show that sorting of TRP-1 and MPR46 was AP-1 dependent, while budding of tyrosinase and Lamp-1 required AP-3. Depletion of clathrin inhibited sorting of all four cargo proteins, suggesting that AP-1 and AP-3 are involved in the formation of distinct types of clathrin-coated vesicles, each of which is characterized by the incorporation of specific cargo membrane proteins." @default.
- W2094037158 created "2016-06-24" @default.
- W2094037158 creator A5012975261 @default.
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- W2094037158 creator A5078374690 @default.
- W2094037158 date "2008-06-28" @default.
- W2094037158 modified "2023-10-18" @default.
- W2094037158 title "AP‐1 and AP‐3 Mediate Sorting of Melanosomal and Lysosomal Membrane Proteins into Distinct Post‐Golgi Trafficking Pathways" @default.
- W2094037158 cites W1485689124 @default.
- W2094037158 cites W1506306229 @default.
- W2094037158 cites W1510734305 @default.
- W2094037158 cites W1536338076 @default.
- W2094037158 cites W1584244824 @default.
- W2094037158 cites W1807798123 @default.
- W2094037158 cites W1860671032 @default.
- W2094037158 cites W1914274337 @default.
- W2094037158 cites W1965381942 @default.
- W2094037158 cites W1967581940 @default.
- W2094037158 cites W1972832014 @default.
- W2094037158 cites W1973583404 @default.
- W2094037158 cites W1975428752 @default.
- W2094037158 cites W1978982003 @default.
- W2094037158 cites W1980269923 @default.
- W2094037158 cites W1986696160 @default.
- W2094037158 cites W1993168711 @default.
- W2094037158 cites W1996770933 @default.
- W2094037158 cites W1999056400 @default.
- W2094037158 cites W2002845578 @default.
- W2094037158 cites W2006736908 @default.
- W2094037158 cites W2007396502 @default.
- W2094037158 cites W2009942743 @default.
- W2094037158 cites W2012848672 @default.
- W2094037158 cites W2019695085 @default.
- W2094037158 cites W2023172957 @default.
- W2094037158 cites W2026590190 @default.
- W2094037158 cites W2030465435 @default.
- W2094037158 cites W2038750623 @default.
- W2094037158 cites W2039979410 @default.
- W2094037158 cites W2043835841 @default.
- W2094037158 cites W2048875689 @default.
- W2094037158 cites W2049657352 @default.
- W2094037158 cites W2052542184 @default.
- W2094037158 cites W2052673946 @default.
- W2094037158 cites W2056283979 @default.
- W2094037158 cites W2071725693 @default.
- W2094037158 cites W2075880910 @default.
- W2094037158 cites W2082700144 @default.
- W2094037158 cites W2084317972 @default.
- W2094037158 cites W2084480802 @default.
- W2094037158 cites W2086341057 @default.
- W2094037158 cites W2089554293 @default.
- W2094037158 cites W2096868964 @default.
- W2094037158 cites W2098485024 @default.
- W2094037158 cites W2104031571 @default.
- W2094037158 cites W2104063456 @default.
- W2094037158 cites W2107292352 @default.
- W2094037158 cites W2112235909 @default.
- W2094037158 cites W2112488154 @default.
- W2094037158 cites W2115912241 @default.
- W2094037158 cites W2115977533 @default.
- W2094037158 cites W2116611793 @default.
- W2094037158 cites W2121357228 @default.
- W2094037158 cites W2122369254 @default.
- W2094037158 cites W2128969723 @default.
- W2094037158 cites W2133324304 @default.
- W2094037158 cites W2133591889 @default.
- W2094037158 cites W2143487345 @default.
- W2094037158 cites W2144953684 @default.
- W2094037158 cites W2145089843 @default.
- W2094037158 cites W2148851012 @default.
- W2094037158 cites W2150187881 @default.
- W2094037158 cites W2152152703 @default.
- W2094037158 cites W2154734157 @default.
- W2094037158 cites W2157272365 @default.
- W2094037158 cites W2158097661 @default.
- W2094037158 cites W2159803769 @default.
- W2094037158 cites W2161900691 @default.
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- W2094037158 cites W2170758609 @default.
- W2094037158 cites W2170832451 @default.
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- W2094037158 doi "https://doi.org/10.1111/j.1600-0854.2008.00745.x" @default.
- W2094037158 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18410487" @default.
- W2094037158 hasPublicationYear "2008" @default.
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