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• W2094054881 abstract "Apoptosis was induced in HeLa cells by exposure to 50 µM N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for various time intervals (up to 120 min). Apoptotic death was confirmed by the microscopic observation of cell blebbing, cell granulation, and cell aggregation. Cells also showed loss of phospholipid symmetry as judged by immunofluorescent microscopy with fluorescently labeled phosphatidyl serine-specific annexin V. In addition, staining of cells with ethidium bromide showed the presence of genomic DNA apoptotic bodies. The protein expression levels of c-jun and c-fos increased in DNA-damaged HeLa cells after MNNG treatment in a time-dependent fashion. Although the levels of c-fos increased rapidly during the first 30 min and remained high for 2 hr, the increase in c-jun expression was more gradual and slower (60–120 min) after MNNG treatment. These results are consistent with the conclusion that c-fos is important in the initial stages (commitment phase) of apoptosis and c-jun is involved in the late stages (execution phase) of apoptosis induced with alkylating agents" @default.
• W2094054881 created "2016-06-24" @default.
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• W2094054881 date "2000-01-01" @default.
• W2094054881 modified "2023-09-27" @default.
• W2094054881 title "Expression of c-<i>jun</i>and c-<i>fos</i>in Apoptotic Cells After DNA Damage" @default.
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• W2094054881 doi "https://doi.org/10.3109/07357900009012203" @default.
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